Modified Lipoproteins by Acrylamide Showed More Atherogenic Properties and Exposure of Acrylamide Induces Acute Hyperlipidemia and Fatty Liver Changes in Zebrafish
| Autor: | So-Mang Lim, Ji-Mi Baek, Jihoe Kim, Kyung-Hyun Cho, Inho Choi, Seong-Min Kim, Jae-Yong Kim |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Time Factors Lipoproteins Hyperlipidemias Toxicology chemistry.chemical_compound Phagocytosis Cell Line Tumor Internal medicine Hyperlipidemia medicine Animals Humans Molecular Biology Cellular Senescence Zebrafish Carcinogen Acrylamide Apolipoprotein A-I Dose-Response Relationship Drug Triglyceride Chemistry Macrophages Fatty liver Lipid metabolism Fibroblasts Atherosclerosis medicine.disease Fatty Liver Lipoproteins LDL Endocrinology Liver Proteolysis Lipoproteins HDL Reactive Oxygen Species Cardiology and Cardiovascular Medicine Cell aging Biomarkers Lipoprotein |
| Zdroj: | Cardiovascular Toxicology. 15:300-308 |
| ISSN: | 1559-0259 1530-7905 |
| DOI: | 10.1007/s12012-014-9294-7 |
| Popis: | Acrylamide is a well-known potent carcinogen and neurotoxin that, until now, has not been sufficiently investigated with regard to its effects on lipid metabolism. We investigated physiological effects of acrylamide (AA) on lipoprotein metabolism using human macrophages, dermal cells, and zebrafish models. Functional and structural properties of lipoproteins were modified by AA (final concentration of 5-100 mM) with loss of antioxidant ability and multimerization of apoA-I in vitro. AA exacerbated LDL oxidation, degradation, and LDL uptake into macrophages with increased ROS production. In human cells, treatment of AA (1-100 μM) caused cellular senescence of dermal cells with severe cytotoxicity. Waterborne exposure of zebrafish in cage water containing AA (300 ppm) resulted in acute death within 26 h along with elevation of body weight, blood glucose, triglyceride, and hepatic inflammation. AA exposure caused fat accumulation in liver in a dose-dependent manner. In conclusion, AA affected lipoprotein metabolism to result exacerbation of atherosclerosis. Exposure of zebrafish to AA resulted in acute inflammatory death with hyperlipidemia. |
| Databáze: | OpenAIRE |
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