Temporal and sequential transcriptional dynamics define lineage shifts in corticogenesis

Autor: Tanzila Mukhtar, Jeremie Breda, Manal A Adam, Marcelo Boareto, Pascal Grobecker, Zahra Karimaddini, Alice Grison, Katja Eschbach, Ramakrishnan Chandrasekhar, Swen Vermeul, Michal Okoniewski, Mikhail Pachkov, Corey C Harwell, Suzana Atanasoski, Christian Beisel, Dagmar Iber, Erik van Nimwegen, Verdon Taylor
Rok vydání: 2022
Předmět:
Cortical development
Lineage specification
Networks
Signaling pathways
Transcriptional landscape
Neurogenesis
Intellectual and Developmental Disabilities (IDD)
1.1 Normal biological development and functioning
Stem Cell Research - Embryonic - Non-Human
Regenerative Medicine
Medical and Health Sciences
General Biochemistry
Genetics and Molecular Biology
Mice
lineage specification
Neural Stem Cells
Underpinning research
Information and Computing Sciences
transcriptional landscape
Genetics
Animals
Humans
Cell Lineage
cortical development
Molecular Biology
Embryonic Stem Cells
Cerebral Cortex
Neurons
General Immunology and Microbiology
General Neuroscience
Infant
Newborn
Neurosciences
Infant
Cell Differentiation
Biological Sciences
Newborn
Stem Cell Research
signaling pathways
Brain Disorders
networks
Neurological
Stem Cell Research - Nonembryonic - Non-Human
Developmental Biology
Zdroj: The EMBO journal, vol 41, iss 24
The EMBO Journal, 41 (24)
ISSN: 1460-2075
0261-4189
Popis: The cerebral cortex contains billions of neurons, and their disorganization or misspecification leads to neurodevelopmental disorders. Understanding how the plethora of projection neuron subtypes are generated by cortical neural stem cells (NSCs) is a major challenge. Here, we focused on elucidating the transcriptional landscape of murine embryonic NSCs, basal progenitors (BPs), and newborn neurons (NBNs) throughout cortical development. We uncover dynamic shifts in transcriptional space over time and heterogeneity within each progenitor population. We identified signature hallmarks of NSC, BP, and NBN clusters and predict active transcriptional nodes and networks that contribute to neural fate specification. We find that the expression of receptors, ligands, and downstream pathway components is highly dynamic over time and throughout the lineage implying differential responsiveness to signals. Thus, we provide an expansive compendium of gene expression during cortical development that will be an invaluable resource for studying neural developmental processes and neurodevelopmental disorders.
The EMBO Journal, 41 (24)
ISSN:0261-4189
ISSN:1460-2075
Databáze: OpenAIRE
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