Temporal and sequential transcriptional dynamics define lineage shifts in corticogenesis
Autor: | Tanzila Mukhtar, Jeremie Breda, Manal A Adam, Marcelo Boareto, Pascal Grobecker, Zahra Karimaddini, Alice Grison, Katja Eschbach, Ramakrishnan Chandrasekhar, Swen Vermeul, Michal Okoniewski, Mikhail Pachkov, Corey C Harwell, Suzana Atanasoski, Christian Beisel, Dagmar Iber, Erik van Nimwegen, Verdon Taylor |
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Rok vydání: | 2022 |
Předmět: |
Cortical development
Lineage specification Networks Signaling pathways Transcriptional landscape Neurogenesis Intellectual and Developmental Disabilities (IDD) 1.1 Normal biological development and functioning Stem Cell Research - Embryonic - Non-Human Regenerative Medicine Medical and Health Sciences General Biochemistry Genetics and Molecular Biology Mice lineage specification Neural Stem Cells Underpinning research Information and Computing Sciences transcriptional landscape Genetics Animals Humans Cell Lineage cortical development Molecular Biology Embryonic Stem Cells Cerebral Cortex Neurons General Immunology and Microbiology General Neuroscience Infant Newborn Neurosciences Infant Cell Differentiation Biological Sciences Newborn Stem Cell Research signaling pathways Brain Disorders networks Neurological Stem Cell Research - Nonembryonic - Non-Human Developmental Biology |
Zdroj: | The EMBO journal, vol 41, iss 24 The EMBO Journal, 41 (24) |
ISSN: | 1460-2075 0261-4189 |
Popis: | The cerebral cortex contains billions of neurons, and their disorganization or misspecification leads to neurodevelopmental disorders. Understanding how the plethora of projection neuron subtypes are generated by cortical neural stem cells (NSCs) is a major challenge. Here, we focused on elucidating the transcriptional landscape of murine embryonic NSCs, basal progenitors (BPs), and newborn neurons (NBNs) throughout cortical development. We uncover dynamic shifts in transcriptional space over time and heterogeneity within each progenitor population. We identified signature hallmarks of NSC, BP, and NBN clusters and predict active transcriptional nodes and networks that contribute to neural fate specification. We find that the expression of receptors, ligands, and downstream pathway components is highly dynamic over time and throughout the lineage implying differential responsiveness to signals. Thus, we provide an expansive compendium of gene expression during cortical development that will be an invaluable resource for studying neural developmental processes and neurodevelopmental disorders. The EMBO Journal, 41 (24) ISSN:0261-4189 ISSN:1460-2075 |
Databáze: | OpenAIRE |
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