The nitrone spin trap PBN alters the cellular response to H2O2: activation of the EGF receptor/ERK pathway
| Autor: | Ippolita Cantuti-Castelevetri, Waleed N Hassan, Natalia A Denisova, K. Eric Paulson, Amy S. Yee, J. A. Joseph |
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| Rok vydání: | 2002 |
| Předmět: |
MAPK/ERK pathway
Src Homology 2 Domain-Containing Transforming Protein 1 Stimulation medicine.disease_cause PC12 Cells Biochemistry Cyclic N-Oxides Physiology (medical) medicine Animals Drug Interactions Phosphorylation Receptor Adaptor Proteins Signal Transducing chemistry.chemical_classification Reactive oxygen species Chemistry Proteins Signal transducing adaptor protein Free Radical Scavengers Hydrogen Peroxide Molecular biology Rats Cell biology Enzyme Activation ErbB Receptors Adaptor Proteins Vesicular Transport Shc Signaling Adaptor Proteins NMDA receptor Calcium Nitrogen Oxides Mitogen-Activated Protein Kinases Signal transduction Oxidative stress |
| Zdroj: | Free Radical Biology and Medicine. 32:551-561 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/s0891-5849(02)00744-x |
| Popis: | The nitrone spin trap PBN has been shown to protect neuronal cells from reactive oxygen species both in culture and in vivo. As an approach to understanding the molecular mechanisms by which PBN may function to protect cells, we examined whether PBN alters the cellular response to reactive oxygen species. H(2)O(2) stimulation of PC-12 cells results in weak activation of both the ERK and JNK signal transduction pathways. PBN pretreatment of PC-12 cells, followed by H(2)O(2) stimulation, results in strong and selective activation of the pro-survival ERK pathway. H(2)O(2) induction of ERK activity in PBN-pretreated cells was shown to be dependent on extracellular Ca(+2) influx. Further analysis of the ERK pathway showed that in PBN-pretreated cells, EGF receptor and the adapter protein SHC were phosphorylated in a Ca(+2)-dependent, ligand-independent manner following H(2)O(2) stimulation. Interestingly, H(2)O(2) stimulation of PBN-pretreated cells results in only 30% of the increase in intracellular Ca(+2) as compared to untreated cells following H(2)O(2) stimulation. These data suggest a model in which PBN attenuates H(2)O(2)-induced Ca(+2) entry, yet magnifies or alters Ca(+2) action, resulting in the activation of the EGF receptor/ERK pathway. |
| Databáze: | OpenAIRE |
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