Transforming Growth Factor β Regulates the Expression of the M2 Muscarinic Receptor in Atrial Myocytes via an Effect on RhoA and p190RhoGAP
| Autor: | Jonas B. Galper, Alexander G. Papageorge, Joey V. Barnett, Simone M. Ward, Jay S. Desgrosellier, Ho-Jin Park, Serban P. Georgescu, Xiaoli Zhuang |
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| Rok vydání: | 2006 |
| Předmět: |
Cell signaling
RHOA Pyridines Cell Stimulation Chick Embryo Biochemistry Transforming Growth Factor beta biology.animal Muscarinic acetylcholine receptor medicine Animals Heart Atria Enzyme Inhibitors Mink Promoter Regions Genetic Molecular Biology Cells Cultured Muscle Cells Receptor Muscarinic M2 biology Kinase Cell Biology Amides Cell biology medicine.anatomical_structure Gene Expression Regulation biology.protein Carrier Proteins rhoA GTP-Binding Protein Signal Transduction Transforming growth factor |
| Zdroj: | Journal of Biological Chemistry. 281:19995-20002 |
| ISSN: | 0021-9258 |
| Popis: | Transforming growth factor beta (TGFbeta) signaling is involved in the development and regulation of multiple organ systems and cellular signaling pathways. We recently demonstrated that TGFbeta regulates the response of atrial myocytes to parasympathetic stimulation. Here, TGFbeta(1) is shown to inhibit expression of the M(2) muscarinic receptor (M(2)), which plays a critical role in the parasympathetic response of the heart. This effect is mimicked by overexpression of a dominant negative mutant of RhoA and by the RhoA kinase inhibitor Y27632, whereas adenoviral expression of a dominant activating-RhoA reverses TGFbeta inhibition of M(2) expression. TGFbeta(1) also mediates a decrease in GTP-bound RhoA and a reciprocal increase in the expression of the RhoA GTPase-activating protein, p190RhoGAP, whereas total RhoA is unchanged. Inhibition of M(2) promoter activity by TGFbeta(1) is mimicked by overexpression of p190RhoGAP, whereas a dominant negative mutant of p190RhoGAP reverses this effect of TGFbeta(1). In contrast to atrial myocytes, in mink lung epithelial cells, in which TGFbeta signaling through activation of RhoA has been previously identified, TGFbeta(1) stimulated an increase in GTP-bound RhoA in association with a reciprocal decrease in the expression of p190RhoGAP. Both effects demonstrated a similar dose dependence on TGFbeta(1). Thus TGFbeta regulation of M(2) muscarinic receptor expression is dependent on RhoA, and TGFbeta regulation of p190RhoGAP expression may be a cell type-specific mechanism for TGFbeta signaling through RhoA. |
| Databáze: | OpenAIRE |
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