Na+-driven ATP synthesis in Methanobacterium thermoautotrophicum can be modulated with sodium ion concentrations in the growth medium
Autor: | Miloslav Greksák, Peter Šmigáň, Ľubica Horovská |
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Jazyk: | angličtina |
Předmět: |
Sodium
ATPase Inorganic chemistry Biophysics chemistry.chemical_element Stimulation Methanogen Na+-translocating ATPase Biochemistry Ion chemistry.chemical_compound (Methanobacterium thermoautotrophicum) Structural Biology Genetics Molecular Biology ATP synthesis Growth medium ATP synthase biology Chemistry Cell Biology biology.organism_classification Methanobacterium thermoautotrophicum biology.protein |
Zdroj: | FEBS Letters. (1):85-88 |
ISSN: | 0014-5793 |
DOI: | 10.1016/0014-5793(88)80990-6 |
Popis: | Methane formation by nongrowing cells of Methanobacterium thermoautotrophicum ΔH can be enhanced with sodium ions only when the cells were originally grown in the presence of a low (5 mM) concentration of NaCl. ATP synthesis in the cells driven by an artificially imposed Na+ gradient can be modulated by changing the sodium ion concentration in the growth medium while H+ gradient-driven ATP synthesis does not depend on it. Stimulation of the entire ATPase activity with Na+ in permeabilized cells depends also on the Na+ concentration in the growth medium. The results obtained might indicate the presence of an inducible Na+-translocating ATPase in Methanobacterium thermoautotrophicum. |
Databáze: | OpenAIRE |
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