Graphene Oxide Functional Nanohybrids with Magnetic Nanoparticles for Improved Vectorization of Doxorubicin to Neuroblastoma Cells
| Autor: | Anna Dubrovska, Francesca Iemma, Gerardo F. Goya, Manuela Curcio, Marion Le Grand, Annafranca Farfalla, Florida Voli, Giuseppe Cirillo, Luigi Lerra, Beatriz Sanz, Silke Hampel, Orazio Vittorio, Fiore Pasquale Nicoletta |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Oxide
Serum albumin Pharmaceutical Science lcsh:RS1-441 02 engineering and technology 010402 general chemistry 01 natural sciences Article law.invention lcsh:Pharmacy and materia medica chemistry.chemical_compound law synergism polycyclic compounds Bioconjugation biology Graphene iron oxide nanoparticles 021001 nanoscience & nanotechnology 0104 chemical sciences nanohybrids magnetic targeting chemistry biology.protein Magnetic nanoparticles graphene oxide Nanocarriers 0210 nano-technology Iron oxide nanoparticles Conjugate Nuclear chemistry |
| Zdroj: | Pharmaceutics, Vol 11, Iss 1, p 3 (2018) Zaguán. Repositorio Digital de la Universidad de Zaragoza instname Pharmaceutics Volume 11 Issue 1 Pharmaceutics 11(2019)1, 3 |
| ISSN: | 1999-4923 |
| Popis: | With the aim to obtain a site-specific doxorubicin (DOX) delivery in neuroblastoma SH-SY5Y cells, we designed an hybrid nanocarrier combining graphene oxide (GO) and magnetic iron oxide nanoparticles (MNPs), acting as core elements, and a curcumin&ndash human serum albumin conjugate as functional coating. The nanohybrid, synthesized by redox reaction between the MNPs@GO system and albumin bioconjugate, consisted of MNPs@GO nanosheets homogeneously coated by the bioconjugate as verified by SEM investigations. Drug release experiments showed a pH-responsive behavior with higher release amounts in acidic (45% at pH 5.0) vs. neutral (28% at pH 7.4) environments. Cell internalization studies proved the presence of nanohybrid inside SH-SY5Y cytoplasm. The improved efficacy obtained in viability assays is given by the synergy of functional coating and MNPs constituting the nanohybrids: while curcumin moieties were able to keep low DOX cytotoxicity levels (at concentrations of 0.44&ndash 0.88 µ M), the presence of MNPs allowed remote actuation on the nanohybrid by a magnetic field, increasing the dose delivered at the target site. |
| Databáze: | OpenAIRE |
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