Der f 2 activates phospholipase D in human T lymphocytes from Dermatophagoides farinae specific allergic individuals: Involvement of protein kinase C-α
| Autor: | Ki-Sung Lee, Ha Baik Lee, Joong-Soo Han, Jae-Won Oh, Yong-Seok Kim, Eun Young Kim, Bon Suk Koo |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Adult
Hypersensitivity Immediate Male Allergy Protein Kinase C-alpha Adolescent T-Lymphocytes Clinical Biochemistry Mite allergen PKC alpha Biochemistry Arthropod Proteins Phospholipase D Animals Humans Medicine Antigens Dermatophagoides Molecular Biology Protein Kinase C Protein kinase C Skin Tests Dermatophagoides farinae business.industry Activator (genetics) Phorbol Myristate Acetate medicine.disease Molecular biology Up-Regulation enzymes and coenzymes (carbohydrates) Cytosol Immunology Tetradecanoylphorbol Acetate Molecular Medicine Female lipids (amino acids peptides and proteins) business |
| Zdroj: | Experimental & Molecular Medicine. 36:486-492 |
| ISSN: | 2092-6413 |
| Popis: | The major house-dust mite allergen, Der f 2, stimulates the phospholipase D (PLD) in T lymphocytes from Dermatophagoides farinae specific allergic individuals. PLD activity increased more than two-fold in T cells from allergic patients compared with those cells from normal controls with maximal responses within 30 min after exposure of Der f 2. A well-known PLD activator PKC-alpha was found to be translocated to membrane from cytosol in Der f 2-treated T cells from Dermatophagoides farinae specific allergic individuals. Down-regulation of PKC-alpha with phorbol myristate acetate pretreatment for 24 h abolished Der f 2-induced PLD activation. Ro 320432, PKC inhibitor also reduced the effects of Der f 2-induced PLD activation suggesting that PKC-alpha acts as upstream activator of PLD in Der f 2-treated T cells. Taken together, the present data suggest that Der f 2 can stimulate PLD activity through the PKC-alpha activation in T cells from Dermatophagoides farinae allergic individuals. |
| Databáze: | OpenAIRE |
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