Development of a droplet digital polymerase chain reaction (ddPCR) assay for the detection of Tomato brown rugose fruit virus (ToBRFV) in tomato and pepper seeds

Autor:	Brenda Yazmín Vargas-Hernández, José Abrahán Ramírez-Pool, Leandro Alberto Núñez-Muñoz, Berenice Calderón-Pérez, Rodolfo De La Torre-Almaráz, Jesús Hinojosa-Moya, Beatriz Xoconostle-Cázares, Roberto Ruiz-Medrano
Rok vydání:	2022
Předmět:	Solanum lycopersicum Fruit Virology Seeds Tobamovirus Polymerase Chain Reaction Plant Diseases
Zdroj:	Journal of Virological Methods. 302:114466
ISSN:	0166-0934
Popis:	In the present study, a droplet digital PCR assay was developed for detection of Tomato brown rugose fruit virus, a new Tobamovirus of tomato and other solanaceous plants, which expands the diagnostic strategies for this pathogen. Candidate reference DNA material was also obtained to be employed as positive control in tomato and pepper samples. Recombinant plasmids encode for ToBRFV coat protein (CP-ToBRFV) gene and Solanum lycopersicum GAPDH fragments, and CP-ToBRFV and Capsicum annuum GAPDH. To our knowledge, this is the first report of ToBRFV detection in tomato and pepper seeds using ddPCR.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::416900674f39099503c7f9f9a43dcd95 https://doi.org/10.1016/j.jviromet.2022.114466 Zobrazit plný text záznamu Full Text from ScienceDirect