Polarized regulatory landscape and Wnt responsiveness underlie Hox activation in embryos
| Autor: | Sander C. Tan, Wouter de Laat, Roel Neijts, Menno P. Creyghton, Shilu Amin, Jacqueline Deschamps, Carina van Rooijen |
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| Přispěvatelé: | Hubrecht Institute for Developmental Biology and Stem Cell Research |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Transcriptional Activation DNA accessibility Biology Regulatory landscapes Transcription initiation 03 medical and health sciences chemistry.chemical_compound Research Communication Mice 0302 clinical medicine Developmental enhancers Transcription (biology) Genetics Journal Article Animals Hox gene Enhancer Homeodomain Proteins Wnt signaling pathway Gene Expression Regulation Developmental Embryo Embryo Mammalian Cell biology Mice Inbred C57BL Wnt Proteins 030104 developmental biology Enhancer Elements Genetic chemistry Chromatin conformation Developmental biology 030217 neurology & neurosurgery DNA Hox regulation Developmental Biology |
| Zdroj: | Genes and Development, 30(17), 1937. Cold Spring Harbor Laboratory Press Genes & Development, 30(17), 1937-42. Cold Spring Harbor Laboratory Press |
| ISSN: | 0890-9369 |
| Popis: | Sequential 3′-to-5′ activation of the Hox gene clusters in early embryos is a most fascinating issue in developmental biology. Neither the trigger nor the regulatory elements involved in the transcriptional initiation of the 3′-most Hox genes have been unraveled in any organism. We demonstrate that a series of enhancers, some of which are Wnt-dependent, is located within a HoxA 3′ subtopologically associated domain (subTAD). This subTAD forms the structural basis for multiple layers of 3′-polarized features, including DNA accessibility and enhancer activation. Deletion of the cassette of Wnt-dependent enhancers proves its crucial role in initial transcription of HoxA at the 3′ side of the cluster. |
| Databáze: | OpenAIRE |
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