Molecular cloning of Kunitz-type proteinase inhibitor group B genes from potato
| Autor: | Palmiro Poltronieri, Pasqua Fasano, Angelo Santino, Anastasia A. Krinitsina, A. B. Shevelev, Anna S. Speranskaya, Tatiana A. Valueva |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Molecular Sequence Data
Sequence alignment Biology Molecular cloning Biochemistry Genome law.invention law Protease Inhibitors Amino Acid Sequence Cloning Molecular Peptide sequence Gene Phylogeny Polymerase chain reaction Plant Proteins Solanum tuberosum Genetics Cloning Base Sequence food and beverages General Medicine Molecular biology genomic DNA Peptides Sequence Alignment |
| Zdroj: | Biochemistry (Moscow). 70:292-299 |
| ISSN: | 1608-3040 0006-2979 |
| DOI: | 10.1007/s10541-005-0114-3 |
| Popis: | Eighteen clones representing copies of four Kunitz-type proteinase inhibitor group B genes (PKPI-B) obtained by polymerase chain reaction cloning of potato (Solanum tuberosum L. cv. Istrinskii) genomic DNA were sequenced and analyzed. Three new genes were found and named PKPI-B1, PKPI-B2, and PKPI-B10: these were represented by five, two, and seven clones, respectively. The remaining four clones corresponded to the formerly characterized PKPI-B9 gene. These data show that at least four PKPI-B encoding genes are harbored in the genome of potato cv. Istrinskii. Their analysis suggests that variability of PKPI-B encoding genes in potato is limited and could be explained by cross-hybridization events in the ancestor forms rather than by random mutagenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|