Experimental Evidence for the Influence of Cumulus-Oocyte-Complexes on Sperm Release from the Porcine Oviductal Sperm Reservoir
| Autor: | Noboru Manabe, Armin Tuchscherer, József Rátky, Klaus P. Brüssow, Helmut Torner |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Ovulation endocrine system Time Factors animal structures Swine media_common.quotation_subject Population Oviducts Biology Insemination Andrology medicine Animals Hyaluronic Acid education Zona Pellucida reproductive and urinary physiology Glycosaminoglycans Ovum media_common Sperm-Ovum Interactions education.field_of_study Models Statistical urogenital system Embryogenesis Anatomy Oocyte Spermatozoa Sperm Sperm Transport medicine.anatomical_structure Oocytes Oviduct Female Animal Science and Zoology |
| Zdroj: | Journal of Reproduction and Development. 52:249-257 |
| ISSN: | 1348-4400 0916-8818 |
| DOI: | 10.1262/jrd.17085 |
| Popis: | In the pig, a temporal relationship is suggested between sperm release from the sperm reservoir (SR) and ovulation, but the mechanism(s) is still under discussion. In two experiments, the influence of transferred ova on the release of SR-spermatozoa at ovulation and the effect of supplementation with non-sulfated glycosaminoglycan hyaluronan (HA) on embryo development and the number of accessory spermatozoa, respectively, were examined. PMSG/hCG primed ovectomized gilts that had previously received endoscopic low-dose insemination into the cranial uterine horn were used as an experimental model. After salpingectomy, tubal segments (ampulla, cranial, and caudal isthmus) were flushed and sperm numbers or respective accessory spermatozoa were counted. In Experiment 1, the distribution of the sperm population was altered in the presence of cumulus-oocyte-complexes (COCs). A higher proportion of spermatozoa was found after transfer of COCs into one oviduct in the ampulla and cranial isthmus segments compared with the controls (17.5 vs. 4.9%, p |
| Databáze: | OpenAIRE |
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