Determination of Periodontal Ligament Cell Viability in Long Shelf-Life Milk
| Autor: | Jason M. Mailhot, Royce R. Runner, James C. McPherson, Thomas B. Buxton, Thomas G. Marino, Frederick R. Liewehr, Lesley A. West |
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| Rok vydání: | 2000 |
| Předmět: |
Time Factors
Cell Survival Periodontal Ligament Cytological Techniques Statistics as Topic Pasteurization Cell Count Food technology Shelf life law.invention Tissue culture stomatognathic system Tap water law Cell Adhesion Animals Humans Periodontal fiber Viability assay Food science General Dentistry Cells Cultured Analysis of Variance Chemistry business.industry Significant difference Water food and beverages Tooth Avulsion Culture Media stomatognathic diseases Milk Food Technology business Cell Division |
| Zdroj: | Journal of Endodontics. 26:699-702 |
| ISSN: | 0099-2399 |
| DOI: | 10.1097/00004770-200012000-00005 |
| Popis: | The purpose of this study was to determine the ability of long shelf-life milk to serve as a temporary storage medium for the maintenance of periodontal ligament (PDL) cell viability on avulsed teeth. PDL cells were plated onto 24-well culture plates and allowed to attach for 24 h. Minimal Essential Medium was replaced with regular pasteurized milk (refrigerated milk), long shelf-life milk (Parmalat), or Save-A-Tooth. Tap water served as the negative control, and Minimal Essential Medium served as the positive control. The tissue culture plates were incubated at 37 degrees C for 1, 2, 4, or 8 h. Cell viability was determined using a cell proliferation assay (CellTiter 96 AQ Assay) and absorbance read at 490 nm. ANOVA indicated that all media performed significantly better than tap water at all time periods. At 8 h, PDL cell viability in regular pasteurized milk and long shelf-life milk were significantly greater than in Save-A-Tooth (por = 0.001). There was no significant difference between regular pasteurized milk and long shelf-life milk at any time period. These results suggest that long shelf-life milk, which has the advantage of not requiring refrigeration, is as effective a storage medium for avulsed teeth as regular pasteurized milk and more effective than Save-A-Tooth. |
| Databáze: | OpenAIRE |
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