High expression of alternative transcript of granzyme M in the mouse retina
| Autor: | Takahiro Suemoto, Shigetaka Yoshida, Manabu Taniguchi, Sadao Shiosaka, Naoyuki Tani, Ichiro Ishimoto |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
genetic structures
Molecular Sequence Data In situ hybridization Biology Gene Expression Regulation Enzymologic Granzymes Retina Mice chemistry.chemical_compound medicine Animals Photoreceptor Cells Amino Acid Sequence RNA Messenger Northern blot Rats Wistar Eye Proteins In Situ Hybridization Messenger RNA Base Sequence General Neuroscience Serine Endopeptidases Alternative splicing Retinal General Medicine Molecular biology eye diseases Rats Alternative Splicing genomic DNA medicine.anatomical_structure chemistry sense organs Granzyme M |
| Zdroj: | Neuroscience Research. 34:115-123 |
| ISSN: | 0168-0102 |
| DOI: | 10.1016/s0168-0102(99)00036-x |
| Popis: | We have isolated cDNAs to two transcripts, granzyme M and alternative granzyme M mRNA from the mouse eye. Analysis of genomic DNA revealed these transcripts were derived from alternative transcription initiations. Northern blot analysis and reverse transcription-polymerase chain reactions revealed that both transcripts were expressed in the eye, though the alternative form was the major type. In situ hybridization studies demonstrated that alternative granzyme M mRNA localized exclusively in the photoreceptor cells in the retina and expressed only after the opening of the eye, suggesting that these transcripts are related to the maintenance of the retinal structure or functions of matured photoreceptor cells rather than the development or differentiation of retinal cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect