Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment
| Autor: | John Ong, Junzhe Zhao, Athina E. Markaki, Alexander W. Justin, Galit Katarivas Levy, James M. MacDonald |
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| Přispěvatelé: | Ong Hui Chong, John [0000-0001-5103-7311], Markaki, Athina [0000-0002-2265-1256], Apollo - University of Cambridge Repository |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
631/45 Hot Temperature Plasma Gases lcsh:Medicine Biocompatible Materials 02 engineering and technology Sodium Chloride Biochemistry Tissue engineering Materials Testing Bovine serum albumin lcsh:Science Extracellular Matrix Proteins Multidisciplinary 639/301 biology Chemistry Biological techniques article Hydrogels 021001 nanoscience & nanotechnology Human serum albumin humanities Drug delivery Self-healing hydrogels embryonic structures 0210 nano-technology 631/61 Biotechnology medicine.drug Biocompatibility Surface Properties Serum albumin Serum Albumin Human complex mixtures Cell Line 03 medical and health sciences medicine Humans Cell Proliferation 631/1647 Osteoblasts Tissue Engineering lcsh:R Albumin technology industry and agriculture Materials science body regions 030104 developmental biology biology.protein Biophysics Microscopy Electron Scanning lcsh:Q 639/638 |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) Scientific Reports |
| Popis: | Funder: Cambridge Commonwealth, European and International Trust; doi: https://doi.org/10.13039/501100003343 Funder: Trinity College, University of Cambridge; doi: https://doi.org/10.13039/501100000727 Funder: Blavatnik Family Foundation; doi: https://doi.org/10.13039/100011643 Funder: Reuben Foundation Funder: Worshipful Council of Engineers Funder: Isaac Newton Trust; doi: https://doi.org/10.13039/501100004815 Albumin-based hydrogels are increasingly attractive in tissue engineering because they provide a xeno-free, biocompatible and potentially patient-specific platform for tissue engineering and drug delivery. The majority of research on albumin hydrogels has focused on bovine serum albumin (BSA), leaving human serum albumin (HSA) comparatively understudied. Different gelation methods are usually employed for HSA and BSA, and variations in the amino acid sequences of HSA and BSA exist; these account for differences in the hydrogel properties. Heat-induced gelation of aqueous HSA is the easiest method of synthesizing HSA hydrogels however hydrogel opacity and poor cell attachment limit their usefulness in downstream applications. Here, a solution to this problem is presented. Stable and translucent HSA hydrogels were created by controlled thermal gelation and the addition of sodium chloride. The resulting bio-inert hydrogel was then subjected to air plasma treatment which functionalised its surface, enabling the attachment of basement membrane matrix (Geltrex). In vitro survival and proliferation studies of foetal human osteoblasts subsequently demonstrated good biocompatibility of functionalised albumin hydrogels compared to untreated samples. Thus, air plasma treatment enables functionalisation of inert heat-derived HSA hydrogels with extracellular matrix proteins and these may be used as a xeno-free platform for biomedical research or cell therapy. |
| Databáze: | OpenAIRE |
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