Temperature modulates stress response in mainstream anammox reactors
| Autor: | Alejandro Palomo, Paul Magyar, Jing Wei, Barth F. Smets, Robert Niederdorfer, Adriano Joss, Helmut Bürgmann, Damian Hausherr |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Applied microbiology
Microbial ecology Transcription Genetic QH301-705.5 Microbial Consortia Medicine (miscellaneous) 010501 environmental sciences 01 natural sciences Article General Biochemistry Genetics and Molecular Biology Water Purification 03 medical and health sciences chemistry.chemical_compound Bioreactors Stress Physiological Ammonium Compounds Ammonium Anaerobiosis Autotroph Biology (General) 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences biology Temperature Biofilm Genomics biology.organism_classification chemistry Microbial population biology Anammox Environmental chemistry Metagenome Sewage treatment Transcriptome General Agricultural and Biological Sciences Genome Bacterial Bacteria |
| Zdroj: | Communications Biology, 4 Communications Biology, Vol 4, Iss 1, Pp 1-12 (2021) Niederdorfer, R, Hausherr, D, Palomo, A, Wei, J, Magyar, P, Smets, B F, Joss, A & Bürgmann, H 2021, ' Temperature modulates stress response in mainstream anammox reactors ', Communications Biology, vol. 4, no. 1, 23 . https://doi.org/10.1038/s42003-020-01534-8 Communications Biology |
| ISSN: | 2399-3642 |
| DOI: | 10.3929/ethz-b-000528462 |
| Popis: | Autotrophic nitrogen removal by anaerobic ammonium oxidizing (anammox) bacteria is an energy-efficient nitrogen removal process in wastewater treatment. However, full-scale deployment under mainstream conditions remains challenging for practitioners due to the high stress susceptibility of anammox bacteria towards fluctuations in dissolved oxygen (DO) and temperature. Here, we investigated the response of microbial biofilms with verified anammox activity to DO shocks under 20 °C and 14 °C. While pulse disturbances of 0.3 mg L−1 DO prompted only moderate declines in the NH4+ removal rates, 1.0 mg L−1 DO led to complete but reversible inhibition of the NH4+ removal activity in all reactors. Genome-centric metagenomics and metatranscriptomics were used to investigate the stress response on various biological levels. We show that temperature regime and strength of DO perturbations induced divergent responses from the process level down to the transcriptional profile of individual taxa. Community-wide gene expression differed significantly depending on the temperature regime in all reactors, and we found a noticeable impact of DO disturbances on genes involved in transcription, translation, replication and posttranslational modification at 20 °C but not 14 °C. Genome-centric analysis revealed that different anammox species and other key biofilm taxa differed in their transcriptional responses to distinct temperature regimes and DO disturbances. Communications Biology, 4 ISSN:2399-3642 |
| Databáze: | OpenAIRE |
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