Reflection phase microscopy using spatio-temporal coherence of light
| Autor: | Taeseok Daniel Yang, Jeon Woong Kang, Youngwoon Choi, Peter T. C. So, Sungsam Kang, Min Gyu Hyeon, Beop Min Kim, Zahid Yaqoob, Poorya Hosseini |
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| Rok vydání: | 2018 |
| Předmět: |
Microscope
Materials science business.industry Video rate Phase contrast microscopy Spectral density 02 engineering and technology Living cell 021001 nanoscience & nanotechnology 01 natural sciences Article Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials law.invention 010309 optics Optics medicine.anatomical_structure law 0103 physical sciences medicine Nuclear membrane 0210 nano-technology business Cytoplasmic Structure Coherence (physics) |
| Zdroj: | Optica. 5:1468 |
| ISSN: | 2334-2536 |
| DOI: | 10.1364/optica.5.001468 |
| Popis: | Many disease states are associated with cellular biomechanical changes as markers. Label-free phase microscopes are used to quantify thermally driven interface fluctuations, which allow the deduction of important cellular rheological properties. Here, the spatio-temporal coherence of light was used to implement a high-speed reflection phase microscope with superior depth selectivity and higher phase sensitivity. Nanometric scale motion of cytoplasmic structures can be visualized with fine details and three-dimensional resolution. Specifically, the spontaneous fluctuation occurring on the nuclear membrane of a living cell was observed at video rate. By converting the reflection phase into displacement, the sensitivity in quantifying nuclear membrane fluctuation was found to be about one nanometer. A reflection phase microscope can potentially elucidate biomechanical mechanisms of pathological and physiological processes. |
| Databáze: | OpenAIRE |
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