An integrated diagnostic setup for the morphological and molecular identification of theCeratitisFAR complex (C. anonae, C. fasciventris, C. rosa,C. quilicii,Diptera, Tephritidae)
| Autor: | Hélène Delatte, Massimiliano Virgilio, J.-H. Daneel, M. De Meyer, Aruna Manrakhan, Kenny Meganck |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0106 biological sciences Genotype 010603 evolutionary biology 01 natural sciences DNA barcoding Species Specificity Tephritidae Animals DNA Barcoding Taxonomic Ceratitis Genotyping Molecular identification biology Ceratitis rosa L60 - Taxonomie et géographie animales General Medicine biology.organism_classification H10 - Ravageurs des plantes 010602 entomology Evolutionary biology Larva Insect Science Microsatellite Female Identification (biology) Agronomy and Crop Science Microsatellite Repeats |
| Zdroj: | BUlletin of entomological research Bulletin of Entomological Research |
| ISSN: | 1475-2670 0007-4853 |
| Popis: | TheCeratitisFAR complex (Diptera, Tephritidae) includes four economically important frugivorous flies (Ceratitis anonae,Ceratitis fasciventris,Ceratitis quilicii,Ceratitis rosa) whose immature stages and adult females cannot be properly resolved through morphological identification. In order to develop a simplified molecular tool for the identification of two of these species (C. rosa,C. quilicii), we selected a subset of six microsatellite markers out of a panel of 16 loci that were previously developed for the molecular differentiation of the taxa within the complex. These six markers were first testedin silicoand then used for the actual genotyping ofC. quiliciiandC. rosa, resulting in the correct identification of all male reference specimens. Here, we propose an integrated morphological and molecular setup for the identification of the four species of the FAR complex. The decision map relies on preliminary DNA barcoding or morphological identification (when possible) to exclude species not belonging to the complex followed by (a) morphological identification of all adult male specimens and femaleC. anonae, (b) molecular identification via a panel of 16 microsatellite markers for immature stages, damaged vouchers and samples potentially including adult femaleC. fasciventris/C. quilicii/C. rosaand (c) molecular identification via a reduced panel of six microsatellite markers for samples including onlyC. quiliciiandC. rosa. This simplified diagnostic setup was profitably implemented in the framework of the ERAfrica fruit fly project and will help correctly identify species within the FAR complex for their early detection and monitoring. |
| Databáze: | OpenAIRE |
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