Development and validation of a UHPLC-ESI-MS/MS method for the quantification of artepillin C in Brazilian green propolis
| Autor: | Ohana Oliveira Zuza da Silva, Lívia Mara Silva, Ademar A. da Silva Filho, Lorena Rodrigues Riani, Lauriene Ricardo Junqueira, Jorge Willian Leandro Nascimento |
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| Rok vydání: | 2021 |
| Předmět: |
Artepillin C
Liquid Chromatography Quality Control Detection limit Chromatography Chemistry Formic acid Electrospray ionization Selected reaction monitoring Pharmaceutical Science Propolis Mass spectrometry RS1-441 Brazilian Green Propolis chemistry.chemical_compound Pharmacy and materia medica Methanol HD9665-9675 Mass Spectrometry Pharmaceutical industry |
| Zdroj: | Revista de Ciências Farmacêuticas Básica e Aplicada, Vol 42 (2021) |
| ISSN: | 2179-443X 1808-4532 |
| DOI: | 10.4322/2179-443x.0727 |
| Popis: | Objectives: The aim of this study was the development and validation of a fast method to quantify artepillin C in green propolis using ultra high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS). Methods: High purity (97.8%) artepillin C was isolated from green propolis using chromatography techniques. Quantification was performed using a C18(2.1 x 100 mm; 1.7 μm) column, gradient of water and methanol (with 0.01% formic acid) as mobile phase, at a flow rate of 0.4 mL/min and 45 ºC in temperature. A mass spectrometer operated in selected reaction monitoring mode to monitor the deprotonated molecular ion of artepillin C (m/z 299) > fragment ion (m/z 200.12). Several parameters such as specificity, linearity, limit of detection (LOD), limit of quantitation (LOQ), precision, accuracy, and robustness were determined. Results: The method was linear in the 50 – 400 μg/mL range (r2 = 0.9906), showing LOD = 10.79 μg/mL and LOQ = 32.70 μg/mL with satisfactory intra-day and inter-day precision with relative standard deviation (RSD %) of 1.9% and 3.4%, respectively. The accuracy showed recovery of 93-104%, the method was robust and artepillin C was quantified in green propolis at 6.51%. Conclusions: The proposed method showed advantages in comparison with other methods, such as short analysis time and high selectivity for artepillin C. |
| Databáze: | OpenAIRE |
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