The i Blood Group Antigen as a Marker for Umbilical Cord Blood-Derived Mesenchymal Stem Cells
| Autor: | Saara Laitinen, Tero Satomaa, Annamari Heiskanen, Juhani Saarinen, Sari Tiitinen, Leena Valmu, Annika Kotovuori, Ilja Ritamo, Tia Hirvonen, Heidi Anderson, Jarkko Räbinä, Pertti Sistonen, Suvi Natunen, Heli Suila |
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| Rok vydání: | 2012 |
| Předmět: |
Glycan
Galectin 3 Cellular differentiation Cell Biology Ligands N-Acetylglucosaminyltransferases Mass Spectrometry Epitope Flow cytometry Epitopes medicine Humans Stem Cell Niche Galectin medicine.diagnostic_test Gene Expression Profiling Mesenchymal stem cell Amino Sugars Cell Differentiation Mesenchymal Stem Cells I Blood-Group System Cell Biology Hematology Fetal Blood Flow Cytometry Molecular biology Cell biology medicine.anatomical_structure biology.protein Biomarkers Ex vivo Developmental Biology |
| Zdroj: | Stem Cells and Development. 21:455-464 |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2011.0405 |
| Popis: | Multipotent mesenchymal stem cells (MSCs) offer great promise for future regenerative and anti-inflammatory therapies. However, there is a lack of methods to quickly and efficiently isolate, characterize, and ex vivo expand desired cell populations for therapeutic purposes. Single markers to identify cell populations have not been characterized; instead, all characterizations rely on panels of functional and phenotypical properties. Glycan epitopes can be used for identifying and isolating specific cell types from heterogeneous populations, on the basis of their cell-type specific expression and prominent cell surface localization. We have now studied in detail the cell surface expression of the blood group i epitope (linear poly-N-acetyllactosamine chain) in umbilical cord blood (UCB)-derived MSCs. We used flow cytometry and mass spectrometric glycan analysis and discovered that linear poly-N-acetyllactosamine structures are expressed in UCB-derived MSCs, but not in cells differentiated from them. We further verified the findings by mass spectrometric glycan analysis. Gene expression analysis indicated that the stem-cell specific expression of the i antigen is determined by β3-N-acetylglucosaminyltransferase 5. The i antigen is a ligand for the galectin family of soluble lectins. We found concomitant cell surface expression of galectin-3, which has been reported to mediate the immunosuppressive effects exerted by MSCs. The i antigen may serve as an endogenous ligand for this immunosuppressive agent in the MSC microenvironment. Based on these findings, we suggest that linear poly-N-acetyllactosamine could be used as a novel UCB-MSC marker either alone or within an array of MSC markers. |
| Databáze: | OpenAIRE |
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