Differential expression of mRNAs for endothelin-related proteins in human endometrium, myometrium and leiomyoma
| Autor: | Fredrika Pekonen, Tuulikki Nyman, E.-M. Rutanen |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
DNA Complementary Molecular Sequence Data Uterus Gene Expression Biology Endometrium Polymerase Chain Reaction Biochemistry Andrology Endocrinology Internal medicine Gene expression medicine Humans RNA Messenger Receptor Molecular Biology Neprilysin reproductive and urinary physiology Messenger RNA Base Sequence Leiomyoma Receptors Endothelin urogenital system Endothelins Myometrium musculoskeletal system medicine.disease female genital diseases and pregnancy complications medicine.anatomical_structure Uterine Neoplasms cardiovascular system Female |
| Zdroj: | Molecular and Cellular Endocrinology. 103:165-170 |
| ISSN: | 0303-7207 |
| Popis: | The expression of mRNAs encoding endothelin-1 (ET-1) and its receptors (ETA-R and ETB-R) as well as the ET degrading enzyme, neutral endopeptidase 24.11 (NEP), was determined in tissue samples of endometrium, myometrium and leiomyoma by using a reverse transcriptase polymerase chain reaction (RT-PCR) technique. ET-1 mRNA was detected in all samples studied. The level of ET-1 mRNA was higher in endometrium than in myometrium (p < 0.01) and leiomyoma (p < 0.001). The ETA-R mRNA was more abundant in endometrium than in myometrium (p < 0.001). For ETB-R mRNA there was no difference between these tissues. In contrast to ETA-R mRNA, which was more abundant in leiomyoma than in myometrium (p < 0.01), the ETB-R mRNA was less abundant in leiomyoma (p < 0.01). The NEP mRNA was detected in all endometrial samples but not in myometrium and leiomyoma. Our results show that the expression and relative levels of mRNAs encoding ET-1, ETA-R, ETB-R, and NEP vary in different tissue compartments of the human uterus. Since the net biological action of ET-1 in a particular cell type presumably depends on the balance between the peptide itself, its receptors and degrading enzymes, these results suggest different roles for ET-1 action in uterine endometrium, myometrium and leiomyoma. The difference in relative abundance of ETA-R and ETB-R mRNAs between myometrium and leiomyoma suggests that an altered ET-R gene expression may be a contributing factor in myomal growth. |
| Databáze: | OpenAIRE |
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