Peptide-mediated targeting of the islets of Langerhans
| Autor: | Michael J. McGuire, Kausar N. Samli, Christopher B. Newgard, Kathlynn C. Brown, Stephen Albert Johnston |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Endocrinology Diabetes and Metabolism Molecular Sequence Data Peptide Biology Cell Line Rats Sprague-Dawley Islets of Langerhans In vivo Peptide Library Internal medicine Cell Line Tumor Internal Medicine medicine Animals Amino Acid Sequence Peptide library Peptide sequence Insulinoma chemistry.chemical_classification medicine.disease Cell biology Rats Pancreatic Neoplasms Endocrinology medicine.anatomical_structure chemistry Cell culture Female Pancreas Peptides Ex vivo |
| Zdroj: | Diabetes. 54(7) |
| ISSN: | 0012-1797 |
| Popis: | Strategies for restoring β-cell function in diabetic patients would be greatly aided by the ability to target genes, proteins, or small molecules specifically to these cells. Furthermore, the ability to direct imaging agents specifically to β-cells would facilitate diagnosis and monitoring of disease progression. To isolate ligands that can home to β-cells in vivo, we have panned a random phage-displayed 20-mer peptide library on freshly isolated rat islets. We have isolated two 20-mer peptides that bind to islets ex vivo. One of these peptides preferentially homes to the islets of Langerhans in a normal rat with clear differentiation between the endocrine and exocrine cells of the pancreas. Furthermore, this peptide does not target β-cells in a type 2 diabetes animal model, suggesting that the peptide can discriminate between glucose-stimulated insulin secretion–functional and -dysfunctional β-cells. |
| Databáze: | OpenAIRE |
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