Molecular Epidemiology of Drug-Resistant Mycobacterium Tuberculosis in Japan

Autor: Yoshikazu Inoue, Teruo Kirikae, Reiko Kiritani, Noritaka Yamada, Masahide Horiba, Noriko Kawata, Akiko Ichinose, Ken Ohta, Tohru Miyoshi-Akiyama, Nobuyuki Kobayashi, Fuminori Mizukoshi, Kazunari Tsuyuguchi, Fumiko Kirikae, Keiji Funatogawa
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
Drug resistance
0302 clinical medicine
Japan
Drug Resistance
Multiple
Bacterial
Epidemiology
Tuberculosis
Multidrug-Resistant
030212 general & internal medicine
Phylogeny
Aged
80 and over
education.field_of_study
biology
Isoniazid
Middle Aged
QR1-502
whole-genome sequencing
Epidemiological Monitoring
Female
Rifampin
medicine.drug
Research Article
Adult
DNA
Bacterial
medicine.medical_specialty
Tuberculosis
Adolescent
foreign born
030106 microbiology
Population
Emigrants and Immigrants
Microbial Sensitivity Tests
Microbiology
Mycobacterium tuberculosis
03 medical and health sciences
Young Adult
Internal medicine
medicine
Humans
education
Molecular Biology
Antibiotics
Antitubercular
Aged
Molecular epidemiology
Whole Genome Sequencing
business.industry
drug-resistant M. tuberculosis
medicine.disease
biology.organism_classification
business
Rifampicin
Genome
Bacterial
Zdroj: mSphere, Vol 6, Iss 4 (2021)
mSphere
ISSN: 2379-5042
DOI: 10.1128/mSphere.00978-20
Popis: Clinical isolates of drug-resistant (isoniazid and/or rifampicin-resistant) Mycobacterium tuberculosis were obtained from 254 patients diagnosed with drug-resistant tuberculosis in Japan from April 2015 to March 2017 in National Hospital Organization hospitals. The 254 patients were approximately 32% of all 795 patients who were diagnosed with culture-confirmed drug-resistant tuberculosis from 2015 to 2016 nationwide in Japan. The whole-genome sequences of all the isolates from the 254 patients and the lineages of these isolates were determined, and phylogenetic trees were constructed based on single nucleotide polymorphism concatemers. Of these patients, 202 (79.5%) were born in Japan and 52 (20.5%) were born elsewhere. Of the 254 drug-resistant isolates, 54 (21.3%) were multidrug resistant, being resistant to both isoniazid and rifampicin. The percentages of multidrug-resistant isolates were significantly higher in foreign-born (38.5% [20/52]) than Japanese-born patients (16.8% [34/202]). Of the 54 multidrug-resistant isolates, nine were extensively drug resistant, which were all obtained from Japanese-born patients. Five extensively drug-resistant isolates were obtained from patients with incipient tuberculosis. A significant number of multidrug-resistant M. tuberculosis strains were isolated from foreign-born patients from Asian countries that have a high tuberculosis burden. Foreign-derived isolates affect the nationwide genetic diversity of drug-resistant M. tuberculosis in Japan. Extensively drug-resistant M. tuberculosis isolates were transmitted among the Japanese population. IMPORTANCE The incidence rate of tuberculosis (TB) in Japan was 11.5 per 100,000 of the population in 2019. Of TB patients in Japan, 61.1% were aged >70 years, and 10.7% were born outside Japan, mostly in Asian countries with a high burden of tuberculosis. Of the tuberculosis patients in the present study, 5.4% and 1.0% showed resistance to isoniazid and rifampicin, respectively, and 0.7% were multidrug resistant. The objective of this study was to clarify the molecular epidemiological properties of drug-resistant tuberculosis in Japan. Molecular epidemiology provides several clues to inform potential measures to control drug-resistant tuberculosis in Japan.
Databáze: OpenAIRE
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