Role of epithelial sodium channel in the realization of homeostatic effects of aldosterone on the volume of principal cells of cortical collecting ducts in rats after hypoosmotic stress
| Autor: | N. S. Logvinenko, E. I. Solenov, L. N. Ivanova |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Epithelial sodium channel
medicine.medical_specialty General Biochemistry Genetics and Molecular Biology Amiloride Tissue Culture Techniques chemistry.chemical_compound Osmotic Pressure Internal medicine medicine Epithelial Sodium Channel Blockers Animals Kidney Tubules Collecting Rats Wistar Epithelial Sodium Channels Aldosterone Cell Size Fluorescent Dyes Kidney Ion Transport Osmolar Concentration Sodium General Medicine Fluoresceins Epithelial sodium channel blocker Rats Calcein Endocrinology medicine.anatomical_structure chemistry Microscopy Fluorescence Biophysics Homeostasis Intracellular medicine.drug |
| Zdroj: | Bulletin of experimental biology and medicine. 155(5) |
| ISSN: | 1573-8221 |
| Popis: | The mechanism of aldosterone effects (10 nM) on the volume of the principal cells of cortical collecting ducts after hypoosmotic stress (280/140 mOsm/kg) was studied using fluorescence microscopy. The experiments with intracellular fluorescent dye calcein showed that aldosterone significantly decreases the amplitude and rate of increase in principal cell volume during hypoosmotic stress. Epithelial sodium channel blocker amiloride (10(-5)M) significantly attenuated the effects of aldosterone on the amplitude and rate of changes in cell volume. The obtained data attest to the contribution of epithelial sodium channel to the realization of rapid non-genomic effects of aldosterone on the amplitude and rate of changes in volume of the principal cells of cortical collecting ducts in rat kidney after hypoosmotic stress. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink