Minimising instilled volume reduces the impact of fluorescein on clinical measurements of tear film stability
| Autor: | Jennifer K. Mooi, Andreas Müller, Michael T.M. Wang, Jennifer P. Craig, Joevy Lim |
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| Rok vydání: | 2016 |
| Předmět: |
Pairwise correlation
Adult Male medicine.medical_specialty Adolescent Break up time 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine Ophthalmology medicine Humans Fluorescein strip 030212 general & internal medicine Prospective Studies Fluorescein Fluorescent Dyes Cross-Over Studies Dose-Response Relationship Drug General Medicine eye diseases Surgery Instillation Drug chemistry Volume (thermodynamics) Tears 030221 ophthalmology & optometry Dry Eye Syndromes Female Ophthalmic Solutions Optometry |
| Zdroj: | Contact lensanterior eye : the journal of the British Contact Lens Association. 40(3) |
| ISSN: | 1476-5411 |
| Popis: | To compare clinical tear film break-up time measurements obtained non-invasively, with those measured following minimal and conventional volumes of fluorescein instillation.Forty-one subjects (20 male, 21 female, mean±SD age 34±11years), with or without dry eye, participated in a prospective cross-over study. Tear film break-up time was measured by the Tearscope Plus™ with fine grid insert. Measurements were made in triplicate, with no fluorescein instillation (NIBUT), then following application of a minimal volume of 1μl fluorescein from the Dry Eye Test™ (mTBUT), and finally with 15-30μl of fluid instilled via a conventional fluorescein strip (TBUT). A fifteen-minute interval between each set of measurements minimised the risk of residual contamination effects.All three techniques displayed statistically significant pairwise correlation (all p0.001). TBUT values were significantly shorter than both NIBUT (geometric mean 8.6s versus 10.9s, p=0.03) and mTBUT (geometric mean 8.6s versus 10.6s, p=0.03), and demonstrated narrower spread (both p0.05). No significant differences were detected between NIBUT and mTBUT (all p0.05).Tear film break-up time values measured with conventional fluorescein instillation were shortened, while minimal fluorescein instillation and non-invasive methods produced comparable readings. This suggests that minimising instilled volumes can reduce the impact of fluorescein on clinical measurements of tear film stability. |
| Databáze: | OpenAIRE |
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