Evaluation of Estrogenic Activity of Wastewater: Comparison Among In Vitro ERα Reporter Gene Assay, In Vivo Vitellogenin Induction, and Chemical Analysis
| Autor: | Masaru Ihara, Seiichiro Okamoto, Naoyuki Yamashita, Vimal Kumar, Mariko O. Ihara, Yutaka Suzuki, Taisen Iguchi, Chang-Beom Park, Hiroaki Tanaka, Tomokazu Kitamura, Sang-Jung Lee, Shinichi Miyagawa |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Oryzias Estrogen receptor Gene Expression Estrone Vitellogenin chemistry.chemical_compound Vitellogenins In vivo Internal medicine medicine Environmental Chemistry Animals Reporter gene biology Chemistry fungi Estrogen Receptor alpha Estriol Estrogens General Chemistry biology.organism_classification Molecular biology In vitro Endocrinology biology.protein Biological Assay hormones hormone substitutes and hormone antagonists Water Pollutants Chemical |
| Zdroj: | Environmental sciencetechnology. 49(10) |
| ISSN: | 1520-5851 |
| Popis: | The in vitro estrogen receptor (ER) reporter gene assay has long been used to measure estrogenic activity in wastewater. In a previous study, we demonstrated that the assay represents net estrogenic activity in the balance between estrogenic and antiestrogenic activities in wastewater. However, it remained unclear whether the net estrogenic activity measured by the in vitro ERα reporter gene assay can predict the in vivo estrogenic effect of wastewater. To determine this, we measured the following: estrogenic and antiestrogenic activities of wastewater and reclaimed water by the in vitro ERα reporter gene assay, expression of vitellogenin-1 (vtg1) and choriogenin-H (chgH) in male medaka (Oryzias latipes) by quantitative real-time PCR, and estrone, 17β-estradiol, estriol, and 17α-ethynylestradiol concentrations chemically to predict estrogenic activity. The net estrogenic activity measured by the in vitro medaka ERα reporter gene assay predicted the in vivo vtg1/chgH expression in male medaka more accurately than the concentrations of estrogens. These results also mean that in vivo vtg1/chgH expression in male medaka is determined by the balance between estrogenic and antiestrogenic activities. The in vitro medaka ERα reporter gene assay also predicted in vivo vtg1/chgH expression on male medaka better than the human ERα reporter gene assay. |
| Databáze: | OpenAIRE |
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