Polymorphic microsatellite markers for population analysis of a tephritid pest species, Bactrocera tryoni
| Autor: | M. W. Kinnear, H. S. Bariana, M. Frommer, J. A. Sved |
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| Rok vydání: | 1998 |
| Předmět: |
Population
Population genetics Biology Polymerase Chain Reaction law.invention Gene Frequency law Genetics Polymorphic Microsatellite Marker Bactrocera Animals Genomic library education Ecology Evolution Behavior and Systematics Polymerase chain reaction Alleles Ecosystem DNA Primers Bactrocera tryoni education.field_of_study Polymorphism Genetic Base Sequence Diptera biology.organism_classification Genetics Population Microsatellite Queensland Microsatellite Repeats |
| Zdroj: | Molecular ecology. 7(11) |
| ISSN: | 0962-1083 |
| Popis: | To obtain a set of microsatellite markers for the Queensland fruit fly Bactrocera tryoni, a genomic library was screened with a number of simple repeat oligonucleotide probes. Sequencing recovered 22 repeat loci. The microsatellite sequences were short, with repeat numbers ranging from five to 11. Of these, 16 polymerase chain reaction (PCR) primer sets yielded amplifiable products, which were tested on 53 flies from five widely separated sites. All loci showed polymorphism in the population sample, with the number of alleles ranging from two to 16. Several dinucleotide repeats showed alleles separated by single-base differences and multiple steps, suggesting a mutation process more complex than the stepwise mutation model. |
| Databáze: | OpenAIRE |
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