Quantitative Analysis of SecYEG-Mediated Insertion of Transmembrane α-Helices into the Bacterial Inner Membrane
| Autor: | Gunnar von Heijne, Karin Öjemalm, Salomé Calado Botelho, Chiara Stüdle |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Sec61
transmembrane helix SecYEG Biology Polar membrane 03 medical and health sciences 0302 clinical medicine Structural Biology Escherichia coli membrane protein Integral membrane protein Molecular Biology 030304 developmental biology 0303 health sciences Escherichia coli Proteins Cell Membrane Peripheral membrane protein Membrane Proteins Membrane transport Transmembrane protein Cell biology Protein Transport Membrane protein Translocase of the inner membrane Thermodynamics Hydrophobic and Hydrophilic Interactions SEC Translocation Channels 030217 neurology & neurosurgery leader peptidase |
| Zdroj: | Journal of Molecular Biology. 425(15):2813-2822 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/j.jmb.2013.04.025 |
| Popis: | Most integral membrane proteins, both in prokaryotic and eukaryotic cells, are co-translationally inserted into the membrane via Sec-type translocons: the SecYEG complex in prokaryotes and the Sec61 complex in eukaryotes. The contributions of individual amino acids to the overall free energy of membrane insertion of single transmembrane α-helices have been measured for Sec61-mediated insertion into the endoplasmic reticulum (ER) membrane (Nature 450:1026–1030) but have not been systematically determined for SecYEG-mediated insertion into the bacterial inner membrane. We now report such measurements, carried out in Escherichia coli. Overall, there is a good correlation between the results found for the mammalian ER and the E. coli inner membrane, but the hydrophobicity threshold for SecYEG-mediated insertion is distinctly lower than that for Sec61-mediated insertion. |
| Databáze: | OpenAIRE |
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