Tissue inhibitors of metalloproteinases-1 (TIMP-1) and -2(TIMP-2) are major serum factors that stimulate the TIMP-1 gene in human gingival fibroblasts
| Autor: | Kyoko Yamashita, Wan-Qian Zhao, Chihiro Kondo, Xiao-Kui Guo, Takanori Aoki, Taro Hayakawa, Nagahiro Shimojo |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Time Factors
Proto-Oncogene Proteins c-jun Tissue inhibitor of metalloproteinases-1 (TIMP-1) c-jun c-fos and c-ets-1 mRNAs Gingiva Stimulation Biology Matrix metalloproteinase Proto-Oncogene Protein c-ets-1 TIMP-2 Humans Electrophoretic mobility shift assay Secretion RNA Messenger Molecular Biology Cells Cultured Tissue Inhibitor of Metalloproteinase-2 Messenger RNA Binding Sites Tissue Inhibitor of Metalloproteinase-1 c-jun Human gingival fibroblasts (Gin-1 cells) Cell Biology Fibroblasts Cell cycle Tissue inhibitor of metalloproteinase AP-1 Molecular biology Culture Media Transcription Factor AP-1 Gene Expression Regulation Dactinomycin Puromycin Proto-Oncogene Proteins c-fos |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1763(3):296-304 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/j.bbamcr.2006.02.012 |
| Popis: | We demonstrate in this study that both TIMP-1 and TIMP-2 are major serum factors that stimulate the induction of TIMP-1 mRNA in quiescent human gingival fibroblasts (Gin-1 cells) at mid-G1 (6–9 h after serum stimulation) of the cell cycle, but not that of TIMP-2. When we chased the secretion of both TIMP proteins into culture medium containing 10% FCS freed of both TIMPs, TIMP-2 secretion rose to the level in 10% FCS after 24 h, but TIMP-1 secretion remained at a fairly low level even after 3 days, thus reflecting a contrastive difference in the induction of both TIMP mRNAs. The stimulating activity of TIMP-1 on the expression of the TIMP-1 gene switched over to inhibitory activity, when the TIMP-1 concentration in the culture medium exceeded about 30 ng/ml. The depletion of TIMP-1 and TIMP-2 from FCS affected remarkably the induction of c-jun and c-fos mRNAs, but not that of c-ets-1 mRNA. TIMP-1 and TIMP-2-dependent expression of AP-1 protein was further demonstrated by using nuclear extracts of Gin-1 cells in an electrophoretic mobility shift assay. |
| Databáze: | OpenAIRE |
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