Restoration of muscle fibers and satellite cells after isogenic MSC transplantation with microdystrophin gene delivery
| Autor: | Jiqing Cao, Mei-juan Yu, Cheng Zhang, Xin-ming Song, Yingyin Liang, Shan-wei Feng, Fei Chen |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Satellite Cells
Skeletal Muscle Duchenne muscular dystrophy Muscle Fibers Skeletal Biophysics Gene delivery Biology Mesenchymal Stem Cell Transplantation Biochemistry Dystrophin Mice medicine Animals Humans Molecular Biology Mesenchymal stem cell Gene Transfer Techniques Skeletal muscle Genetic Therapy Cell Biology medicine.disease Muscular Dystrophy Duchenne Transplantation Disease Models Animal Retroviridae medicine.anatomical_structure Immunology Cancer research biology.protein Desmin Stem cell |
| Zdroj: | Biochemical and Biophysical Research Communications. 419:1-6 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2012.01.029 |
| Popis: | Duchenne muscular dystrophy is the most prevalent inheritable muscle disease. Transplantation of autologous stem cells with gene direction is an ideal therapeutic approach for the disease. The current study aimed to investigate the restoration of myofibers in mdx mice after mdx bone marrow-derived mesenchymal stem cell (mMSC) transplantation with human microdystrophin delivery. Possible mechanisms of action were also studied. In our research, mMSCs were successfully transduced by retrovirus carrying a functional human microdystrophin gene. Transplantation of transduced mMSCs enabled persistent dystrophin restoration in the skeletal muscle of mdx mice up to the 12th week after transplantation. Simultaneous coexpression of human microdystrophin and desmin showed that implanted mMSCs are capable of long-term survival as muscle satellite cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect