Surface Acoustic Wave Biosensors for the Quantification of TNF-α/SPD-304 Interaction
| Autor: | Chouki Zerrouki, M. Sylla-Iyarreta Veitía, Jean-François Zagury, Nourdin Yaakoubi, Marc Port, Najla Fourati, Hadley Mouhsine, G. Moreau, Matthieu Montes |
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| Přispěvatelé: | Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École normale supérieure - Rennes (ENS Rennes)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire génomique, bioinformatique et applications (GBA), Conservatoire National des Arts et Métiers [CNAM] (CNAM), Laboratoire de Chimie moléculaire, génie des procédés chimiques et énergétiques (CMGPCE), Laboratoire d'Acoustique de l'Université du Mans (LAUM), Centre National de la Recherche Scientifique (CNRS)-Le Mans Université (UM) |
| Rok vydání: | 2016 |
| Předmět: |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
SPD-304 Analytical chemistry 02 engineering and technology 010402 general chemistry Kinetic energy 01 natural sciences mental disorders [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Engineering(all) SAW biosensor Detection limit Chromatography Chemistry Surface acoustic wave General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences DMSO cosolvent Solvent Dissociation constant Affinity TNF-α Gravimetric analysis 0210 nano-technology Saturation (chemistry) Biosensor |
| Zdroj: | Procedia Engineering Eurosensor 2016 Eurosensor 2016, Sep 2016, Budapest, Hungary. pp.432-435, ⟨10.1016/j.proeng.2016.11.537⟩ |
| ISSN: | 1877-7058 |
| DOI: | 10.1016/j.proeng.2016.11.537 |
| Popis: | International audience; In this study, a surface acoustic wave (SAW) biosensor has been investigated to quantify the affinity and to estimate the binding kinetic between tumor necrosis factor α (TNF-α) and its inhibitor: SPD-304. To the best of our knowledge this is first ever report on real time and label free monitoring of TNF-α/ SPD-304 affinity. Effects of the SPD-304 solvent and DMSO cosolvent on TNFα/SPD-304 interaction have been investigated. Gravimetric results indicate that the limit of detection (LOD) of the developed biosensor was of order of 10 nM and that DMSO cosolvent influences the kinetic of interaction, the saturation value, and thus the value of the dissociation constant (Kd) of the TNF-α / SPD-304 system. |
| Databáze: | OpenAIRE |
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