28-day inhalation toxicity of 3-methoxybutyl chloroformate in rats
| Autor: | Hyeon-Yeong Kim, Eun-Sang Cho |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
REACH
Registration Evaluation Authorization and Restriction of Chemicals Health Toxicology and Mutagenesis Physiology MCHC mean corpuscular hemoglobin concentration 010501 environmental sciences MCV mean corpuscular volume Toxicology 01 natural sciences RDW red cell distribution width HGB hemoglobin concentration SPF specific-pathogen-free 0302 clinical medicine MNPCE micronucleated polychromatic erythrocytes Medicine ANOVA analysis of variance Inhalation Sprague Dawley rats GHS Globally Harmonized System of Classification and Labelling of Chemicals GLP Good Laboratory Practice CT computed tomography medicine.anatomical_structure 3-MBCF 3-methoxy butyl chloroformate Toxicity Micronucleus test Inhalation toxicity medicine.medical_specialty No-observed-adverse-effect level MCH mean corpuscular hemoglobin NOAEL no observed adverse effect level MOE The Ministry of Environment Article EDTA ethylenediamine tetraacetic acid NCE normochromatic erythrocytes 03 medical and health sciences HCT hematocrit lcsh:RA1190-1270 PCE polychromatic erythrocytes WBC white blood cell counts OECD Organization for Economic Cooperation and Development 3-Methoxybutyl chloroformate ComputingMethodologies_COMPUTERGRAPHICS 0105 earth and related environmental sciences lcsh:Toxicology. Poisons RBC red blood cell counts 3-methoxybutyl chloroformate business.industry PLT platelets SD Sprague-Dawley occupational hazard Histopathology Bone marrow business 030217 neurology & neurosurgery Respiratory tract |
| Zdroj: | Toxicology Reports, Vol 5, Iss, Pp 213-219 (2018) Toxicology Reports |
| ISSN: | 2214-7500 |
| Popis: | Graphical abstract Highlights • 3-MBCF up to 6 ppm induced mortality in 28-day inhalation study of rats. • The NOAEL of 3-MBCF in 28 day inhalation toxicity study, was less than 3 ppm. • 3-MBCF under 12 ppm did not induce micronucleus formation in the bone marrow of rats. The 28-day repeated inhalation study was applied for hazard assessment of 3-methoxybutyl chloroformate (3-MBCF) in Sprague Dawley rats. Groups of five rats per sex were exposed 6 h/day, 5 days per week for 4 weeks to test substance concentration (ranging from 3 to 12 ppm) using a whole-body exposure system. At the terminal sacrifice, following blood collection and gross pathological examination, organ weights were determined and fixed organs were examined. The micronucleus test was performed using bone marrow cells. Exposure of 3-MBCF induced mortality at concentrations above 6 ppm. Decreases in body weight and food intake, hematologic alterations, organ weight changes, and gross and microscopic findings were seen even at the lowest concentrations of 3 ppm. Histopathology revealed principal test substance exposure correlated with lesions in the respiratory tract in both male and female rats above 3 ppm. Groups of male rats exposed above 6 ppm show microscopic lesions in spleens, livers, testes and epididymides; however, the micronucleated polychromatic erythrocytes frequency in bone marrow cells was not changed. Based on histopathology of the respiratory tract and other organs, the no observed adverse effect level (NOAEL) of 3-MBCF in the present study was less than 3 ppm. |
| Databáze: | OpenAIRE |
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