Targeted Analysis of HSP70 Isoforms in Human Spermatozoa in the Context of Capacitation and Motility

Autor: Sarah Grassi, Marie Bisconti, Baptiste Martinet, Vanessa Arcolia, Jean-François Simon, Ruddy Wattiez, Baptiste Leroy, Elise Hennebert
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: International Journal of Molecular Sciences; Volume 23; Issue 12; Pages: 6497
ISSN: 1422-0067
DOI: 10.3390/ijms23126497
Popis: HSP70s constitute a family of chaperones, some isoforms of which appear to play a role in sperm function. Notably, global proteomic studies analysing proteins deregulated in asthenozoospermia, a main cause of male infertility characterized by low sperm motility, showed the deregulation of some HSP70 isoforms. However, to date, no clear trend has been established since the variations in the abundance of HSP70 isoforms differed between studies. The HSPA2 isoform has been reported to play a key role for fertilization, but its deregulation and possible relocation during capacitation, a maturation process making the spermatozoon capable to fertilize an oocyte, is debated in the literature. The aim of the present study was to investigate the fate of all sperm HSP70 isoforms during capacitation and in relation to sperm motility. Using Multiple-Reaction Monitoring (MRM) mass spectrometry, we showed that the relative abundance of all detected isoforms was stable between non-capacitated and capacitated spermatozoa. Immunofluorescence using two different antibodies also demonstrated the stability of HSP70 isoform localization during capacitation. We also investigated spermatozoa purified from 20 sperm samples displaying various levels of total and progressive sperm motility. We showed that the abundance of HSP70 isoforms is not correlated to sperm total or progressive motility and does not vary significantly between astheno-teratozoospermic, normozoospermic, and teratozoospermic samples.
Databáze: OpenAIRE
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