Effect of leptin on the regulation of placental hormone secretion in cultured human placental cells
| Autor: | Rosa Señarís, Ma Angeles Aniel-Quiroga, P. Martul, Ma Angeles Busturia, Jaime Algorta, Raquel Coya |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Leptin
medicine.medical_specialty Estrone Endocrinology Diabetes and Metabolism Placenta Chorionic Gonadotropin Human chorionic gonadotropin Tissue Culture Techniques chemistry.chemical_compound Endocrinology Human placental lactogen Internal medicine medicine Humans Testosterone Placental lactogen reproductive and urinary physiology Cells Cultured Progesterone Estrone secretion Dose-Response Relationship Drug Estradiol Estriol digestive oral and skin physiology Obstetrics and Gynecology Gene Expression Regulation Developmental Placental Lactogen medicine.anatomical_structure chemistry Female Placental Hormones hormones hormone substitutes and hormone antagonists |
| Zdroj: | Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology. 22(11) |
| ISSN: | 0951-3590 |
| Popis: | Placenta is an important source of leptin during pregnancy that contributes to the high plasma leptin levels in pregnant women. Leptin and its functional receptors are synthesized in trophoblast cells that, in turn, secrete gestational hormones supporting a paracrine or autocrine role for leptin in the endocrine activity of the placenta. In the present study we examined the effect of leptin on in vitro release of gestational hormones (human chorionic gonadotropin (hCG), human placental lactogen (hPL), progesterone, estrogens and testosterone) by human term placental cells in culture. Placentas at term were obtained immediately after delivery from mothers with uncomplicated pregnancies. Progesterone, hCG, hPL, estradiol, estrone, estriol and testosterone levels were measured by different assays in culture media of cells maintained in monolayer culture after incubation for 12, 24, 48 or 72 h with leptin or placebo. Incubation with leptin did not modify hCG, hPL, progesterone, estriol and estrone secretion for any of the doses and times assayed. However, leptin led to a dose-dependent decrease in estradiol release. This effect was observed when treatment with recombinant human leptin spanned from 12 to 72 h. At this time an increase in testosterone levels was observed in leptin-treated cells versus placebo. These results indicate that leptin can be considered a gestational hormone implied in the endocrine function of the placenta, with an important role in control of the production of steroid reproductive hormones in placental cells in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |