Flow Cytometry Analysis of Fungal Ploidy
| Autor: | Robert T. Todd, Ann L. Braverman, Anna Selmecki |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antifungal drug Mycology Biology medicine.disease_cause Microbiology Genome Article Flow cytometry 03 medical and health sciences Virology medicine Humans Genome size Genetics Regulation of gene expression Ploidies medicine.diagnostic_test Staining and Labeling Fungi General Medicine Flow Cytometry 030104 developmental biology Mycoses Nucleic acid Parasitology Ploidy Carcinogenesis Software |
| Zdroj: | Current protocols in microbiology. 50(1) |
| ISSN: | 1934-8533 |
| Popis: | Ploidy, the number of sets of homologous chromosomes in a cell, can alter cellular physiology, gene regulation, and the spectrum of acquired mutations. Advances in single-cell flow cytometry have greatly improved the understanding of how genome size contributes to diverse biological processes including speciation, adaptation, pathogenesis, and tumorigenesis. For example, fungal pathogens can undergo whole genome duplications during infection of the human host and during acquisition of antifungal drug resistance. Quantification of ploidy is dramatically affected by the nucleic acid staining technique and the flow cytometry analysis of single cells. Ploidy in fungi is also impacted by samples that are heterogeneous for both ploidy and morphology, and control strains with known ploidy must be included in every flow cytometry experiment. To detect ploidy changes within fungal strains, the following protocol was developed to accurately and dependably interrogate single-cell ploidy. © 2018 by John Wiley & Sons, Inc. |
| Databáze: | OpenAIRE |
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