Coordinated Eph-ephrin signaling guides migration and axon targeting in the avian auditory system
| Autor: | Karina S. Cramer, Michelle R. Allen-Sharpley |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Auditory Pathways
Receptor EphB1 Recombinant Fusion Proteins Green Fluorescent Proteins Chick Embryo Development Biology Chick lcsh:RC346-429 Antibodies 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Cell Movement Neuropil medicine Animals Humans Auditory system Ephrin Axon lcsh:Neurology. Diseases of the nervous system Migration Receptors Eph Family 030304 developmental biology Neurons 0303 health sciences Axon guidance Erythropoietin-producing hepatocellular (Eph) receptor Gene Expression Regulation Developmental Axons Electroporation medicine.anatomical_structure nervous system Brainstem Ephrins Eph receptor Nucleus Neuroscience 030217 neurology & neurosurgery Brain Stem Signal Transduction Research Article |
| Zdroj: | Allen-Sharpley, Michelle R; & Cramer, Karina S. (2012). Coordinated Eph-ephrin signaling guides migration and axon targeting in the avian auditory system. Neural Development, 7(1), 29. doi: http://dx.doi.org/10.1186/1749-8104-7-29. Retrieved from: http://www.escholarship.org/uc/item/9rs8610w Neural Development Neural Development, Vol 7, Iss 1, p 29 (2012) |
| ISSN: | 1749-8104 |
| DOI: | 10.1186/1749-8104-7-29 |
| Popis: | Background In the avian sound localization circuit, nucleus magnocellularis (NM) projects bilaterally to nucleus laminaris (NL), with ipsilateral and contralateral NM axon branches directed to dorsal and ventral NL dendrites, respectively. We previously showed that the Eph receptor EphB2 is expressed in NL neuropil and NM axons during development. Here we tested whether EphB2 contributes to NM-NL circuit formation. Results We found that misexpression of EphB2 in embryonic NM precursors significantly increased the number of axon targeting errors from NM to contralateral NL in a cell-autonomous manner when forward signaling was impaired. We also tested the effects of inhibiting forward signaling of different Eph receptor subclasses by injecting soluble unclustered Fc-fusion proteins at stages when NM axons are approaching their NL target. Again we found an increase in axon targeting errors compared to controls when forward signaling was impaired, an effect that was significantly increased when both Eph receptor subclasses were inhibited together. In addition to axon targeting errors, we also observed morphological abnormalities of the auditory nuclei when EphB2 forward signaling was increased by E2 transfection, and when Eph-ephrin forward signaling was inhibited by E6-E8 injection of Eph receptor fusion proteins. Conclusions These data suggest that EphB signaling has distinct functions in axon guidance and morphogenesis. The results provide evidence that multiple Eph receptors work synergistically in the formation of precise auditory circuitry. |
| Databáze: | OpenAIRE |
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