Emergence of the mcr-1 colistin resistance gene in extended-spectrum β-lactamase-producing Klebsiella pneumoniae in Taiwan
Autor: | Satowa Suzuki, Yu-Chi Lin, Makoto Kuroda, Jung-Jung Mu |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Microbiology (medical) Klebsiella pneumoniae 030106 microbiology Immunology Taiwan mcr-1 Biology Microbiology Genome beta-Lactamases 03 medical and health sciences 0302 clinical medicine Plasmid Drug Resistance Bacterial medicine Immunology and Allergy 030212 general & internal medicine Klebsiella pneumonia Gene ISApl1 Colistin biology.organism_classification medicine.disease QR1-502 Gene cassette MCR-1 hormones hormone substitutes and hormone antagonists medicine.drug |
Zdroj: | Journal of Global Antimicrobial Resistance, Vol 24, Iss, Pp 278-284 (2021) |
ISSN: | 2213-7165 |
Popis: | Objectives The resistance ofmcr-1-carrying Enterobacteriaceae to polymyxins, which are last-resort antibiotics, has raised great concern worldwide. In this study, four mcr-1-carrying plasmids isolated from Klebsiella pneumoniae clinical isolates were completely sequenced and the genome composition of the mcr-1-carrying plasmids was analysed. Methods Antimicrobial resistance genes and antimicrobial susceptibility of fourmcr-1-carrying K. pneumoniae isolates were characterised. Comparison of mcr-1-carrying plasmids with closely related plasmids and analysis of the mcr-1 gene cassette were performed. Results The genome composition of the fourmcr-1-carrying plasmids revealed the Tn6330 and Tn6390 cassettes embedded in two IncHI1-type plasmids, ΔTn6330 in an IncHI2-type plasmid, and mcr-1–pap2 in an IncX4-type plasmid. We also predicted the intermediate structures of the Tn6330 and Tn6390 cassettes. Conclusion Dissemination of the colistin resistant genemcr-1 in Taiwan could have been driven by various plasmids and mobile gene cassettes. Evolution of the genetic environment has led to diversity in the mcr-1 gene among plasmids. This work sheds light on the urgent need for continued surveillance of the worldwide distribution of mcr-1 and evaluates the public-health risk of colistin resistance. |
Databáze: | OpenAIRE |
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