Sexual dimorphism in human skeletal muscle mitochondrial bioenergetics in response to type 1 diabetes

Autor: Robert Laham, Sofhia V. Ramos, Christopher G. R. Perry, Cynthia M. F. Monaco, Meghan C. Hughes, Catherine A. Bellissimo, Thomas J. Hawke
Rok vydání: 2020
Předmět:
Adult
Male
medicine.medical_specialty
endocrine system diseases
Bioenergetics
Physiology
Endocrinology
Diabetes and Metabolism
Muscle Fibers
Skeletal
030209 endocrinology & metabolism
030204 cardiovascular system & hematology
Mitochondrion
Young Adult
03 medical and health sciences
Sex Factors
0302 clinical medicine
immune system diseases
Physiology (medical)
Internal medicine
Diabetes mellitus
medicine
Humans
Respiratory function
Muscle
Skeletal
Sex Characteristics
Type 1 diabetes
Electron Transport Complex I
Rapid Report
business.industry
Electron Transport Complex II
nutritional and metabolic diseases
Skeletal muscle
Hydrogen Peroxide
medicine.disease
Mitochondria
Sexual dimorphism
Diabetes Mellitus
Type 1
medicine.anatomical_structure
Endocrinology
Case-Control Studies
Calcium
Female
Energy Metabolism
business
Zdroj: Am J Physiol Endocrinol Metab
ISSN: 1522-1555
0193-1849
DOI: 10.1152/ajpendo.00411.2019
Popis: Sexual dimorphism in mitochondrial respiratory function has been reported in young women and men without diabetes, which may have important implications for exercise. The purpose of this study was to determine if sexual dimorphism exists in skeletal muscle mitochondrial bioenergetics in people with type 1 diabetes (T1D). A resting muscle microbiopsy was obtained from women and men with T1D ( n = 10/8, respectively) and without T1D (control; n = 8/7, respectively). High-resolution respirometry and spectrofluorometry were used to measure mitochondrial respiratory function, hydrogen peroxide (mH2O2) emission and calcium retention capacity (mCRC) in permeabilized myofiber bundles. The impact of T1D on mitochondrial bioenergetics between sexes was interrogated by comparing the change between women and men with T1D relative to the average values of their respective sex-matched controls (i.e., delta). These aforementioned analyses revealed that men with T1D have increased skeletal muscle mitochondrial complex I sensitivity but reduced complex II sensitivity and capacity in comparison to women with T1D. mH2O2 emission was lower in women compared with men with T1D at the level of complex I (succinate driven), whereas mCRC and mitochondrial protein content remained similar between sexes. In conclusion, women and men with T1D exhibit differential responses in skeletal muscle mitochondrial bioenergetics. Although larger cohort studies are certainly required, these early findings nonetheless highlight the importance of considering sex as a variable in the care and treatment of people with T1D (e.g., benefits of different exercise prescriptions).
Databáze: OpenAIRE
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