Translational Induction of VEGF Internal Ribosome Entry Site Elements During the Early Response to Ischemic Stress
| Autor: | Leonel Prado-Lourenco, Stéphanie Bornes, Amandine Bastide, Jason S. Iacovoni, Hervé Prats, Anne-Catherine Prats, Eric Lacazette, Christian Touriol, Catherine Zanibellato |
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| Přispěvatelé: | Simon, Marie Francoise, Institut de médecine moléculaire de Rangueil (I2MR), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées- Institut Fédératif de Recherche Bio-médicale Institution (IFR150)-Institut National de la Santé et de la Recherche Médicale (INSERM), MilleGen Prologue Biotech |
| Rok vydání: | 2007 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Physiology Angiogenesis MESH: Codon Initiator Response element Codon Initiator MESH: Base Sequence Mice L Cells Start codon Ischemia MESH: Reverse Transcriptase Polymerase Chain Reaction Protein biosynthesis MESH: Animals MESH: Stress Physiological MESH: L Cells (Cell Line) MESH: Organ Specificity MESH: Muscle Skeletal Reverse Transcriptase Polymerase Chain Reaction MESH: Hindlimb MESH: Gene Expression Regulation Hindlimb Cell biology Organ Specificity MESH: Protein Biosynthesis Acute Disease MESH: 5' Untranslated Regions MESH: Acute Disease Cardiology and Cardiovascular Medicine MESH: Neovascularization Physiologic MESH: Mice Transgenic Molecular Sequence Data MESH: Sequence Alignment Neovascularization Physiologic Mice Transgenic Biology MESH: Sequence Homology Nucleic Acid Vasculogenesis Species Specificity Stress Physiological Sequence Homology Nucleic Acid Animals Humans MESH: Species Specificity RNA Messenger Muscle Skeletal MESH: Mice MESH: RNA Messenger Messenger RNA Reporter gene MESH: Molecular Sequence Data MESH: Humans Base Sequence MESH: Vascular Endothelial Growth Factor A MESH: Male Internal ribosome entry site Gene Expression Regulation Protein Biosynthesis Immunology NIH 3T3 Cells MESH: Ischemia 5' Untranslated Regions Sequence Alignment MESH: NIH 3T3 Cells |
| Zdroj: | Circulation Research Circulation Research, 2007, 100 (3), pp.305-8. ⟨10.1161/01.RES.0000258873.08041.c9⟩ |
| ISSN: | 1524-4571 0009-7330 |
| Popis: | International audience; Vascular endothelial growth factor-A (VEGF), a powerful factor involved in vasculogenesis and angiogenesis, is translationally regulated through 2 independent internal ribosome entry sites (IRESs A and B). IRESs enable an mRNA to be translated under conditions in which 5'-cap-dependent translation is inhibited, such as low oxygen stress. In the VEGF mRNA, IRES A influences translation at the canonical AUG codon, whereas the 5' IRES B element regulates initiation at an upstream, in frame CUG. In this study, we have developed transgenic mice expressing reporter genes under the control of these 2 IRESs. We reveal that although these IRESs display low activity in embryos and adult tissues, they permit efficient translation at early time points in ischemic muscle, a stress under which cap-dependent translation is inhibited. These results demonstrate the in vivo efficacy of the VEGF IRESs in response to a local environmental stress such as hypoxia. |
| Databáze: | OpenAIRE |
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