Enzyme-Linked Immunosorbent Assay of Autoantibodies against Mitochondrial Glycerophosphate Dehydrogenase in Insulin-Dependent and Non-Insulin-Dependent Diabetic Subjects
| Autor: | Josep Vidal, Marta E Fabregat, Monica Gudayol, Carmen Zamorano Benito, Teresa Gallart, Willy Malaisse, Ramon Gomis |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent endocrine system diseases medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Glycerolphosphate Dehydrogenase Autoantigens Biochemistry Epitope Internal medicine medicine Humans Aged Autoantibodies Aged 80 and over chemistry.chemical_classification geography geography.geographical_feature_category biology Insulin Non insulin dependent diabetes mellitus Autoantibody nutritional and metabolic diseases Middle Aged Islet Mitochondria GLYCEROPHOSPHATE DEHYDROGENASE Diabetes Mellitus Type 1 Endocrinology Enzyme Diabetes Mellitus Type 2 Immunoglobulin M chemistry Immunoglobulin G biology.protein Female Antibody |
| Zdroj: | Biochemical and Molecular Medicine. 62:172-177 |
| ISSN: | 1077-3150 |
| DOI: | 10.1006/bmme.1997.2642 |
| Popis: | The mitochondrial enzyme FAD-linked glycerophosphate dehydrogenase (mGDH) plays a key role in the recognition of glucose as a stimulus for insulin release from the pancreatic islet B-cell. In the present study, an ELISA procedure was used for the measurement of mGDH antibodies in both insulin-dependent (IDDM) and non-insulin-dependent (NIDDM) diabetic patients. Positive readings, exceeding the upper limit of the normal range, were recorded in 7 out of 12 IDDM patients, as distinct (P< 0.01) from 2 out of 12 nondiabetic subjects of comparable age. The study conducted in 41 NIDDM patients and 15 control subjects of similar age indicated that the incidence of mGDH-positive cases was not significantly different in the diabetic (4/41) and control (1/15) groups, the measurement of optical density in the positive cases barely exceeding the upper limit of the normal range. These findings indicate that the mitochondrial enzyme mGDH often acts as an antigenic determinant in IDDM, but not in NIDDM, patients. |
| Databáze: | OpenAIRE |
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