Collagen and elastic fibres in acute and chronic liver injury

Autor:	Andrew Hall, Prithi S. Bhathal, Alberto Quaglia, Tu Vinh Luong, Corina Cotoi, Jennifer Watkins
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	0301 basic medicine Adult Liver Cirrhosis Male Pathology medicine.medical_specialty Victoria blue Cirrhosis Science Liver fibrosis Article Extracellular matrix 03 medical and health sciences Young Adult 0302 clinical medicine Medical research medicine Humans Liver diseases Retrospective Studies Multidisciplinary biology Chemistry Liver Failure Acute medicine.disease biology.organism_classification Elastic Tissue Immunohistochemistry Elastin Extracellular Matrix Transplantation Collagen type I alpha 1 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Hepatocyte biology.protein Hepatocytes Medicine Female Collagen
Zdroj:	Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) Scientific Reports
ISSN:	2045-2322
Popis:	The histological distinction between acute and chronic liver injury is a challenging aspect of liver histopathology. It is traditionally based on the interpretation of morphological changes to the extracellular matrix (ECM) at sites of hepatocyte loss using histochemical stains. Our aim was to investigate whether immunohistochemistry and multiplexing for collagen type (I & III) and elastic fibres and a modified Victoria blue method could be helpful. We studied 43 livers removed at transplantation for acute liver failure (ALF, 20 cases) or cirrhosis (23) plus 8 normal controls. In ALF the periportal ECM was normal in 2 cases, contained mainly collagen I associated with a ductular reaction in 6 cases, and delicate elastic strands in 11 cases. Periportal deposition of mainly collagen I and mature elastic fibres was observed in cirrhosis. In ALF the perisinusoidal ECM was intact in 4 cases, collapsed or condensed but of normal composition (predominantly collagen III) in 2 cases, and collapsed and condensed containing mostly collagen I in 17 cases (7 including delicate immature elastic strands). In contrast, bridging fibrous septa of cirrhosis contained abundant collagen 1 and bundles of mature elastin. We propose the use of a scale and the use of immunohistochemistry and multiplexing in additional to histochemical stains to characterise the ECM changes in acute and chronic liver injury.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41398d334f36be95e1c8b85448f98e8e https://doaj.org/article/66f92431e9684711a334667c8e4a59d0 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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