Depolarization-induced release of endocannabinoids by murine dorsal motor nucleus of the vagus nerve neurons differentially regulates inhibitory and excitatory neurotransmission
| Autor: | André Jean, Bruno Lebrun, Jérôme Trouslard, Julien Roux, Nicolas Wanaverbecq |
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| Přispěvatelé: | Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Neurobiologie, Communication Chimique, (LNB), Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Cellulaires et Intégratives (INCI), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
MESH: Capsaicin MESH: 6-Cyano-7-nitroquinoxaline-2 3-dione MESH: Neurons MESH: Endocannabinoids MESH: Receptor Cannabinoid CB1 Kynurenic Acid Receptors Presynaptic Mice 0302 clinical medicine Piperidines Receptor Cannabinoid CB1 MESH: Vagus Nerve MESH: Animals MESH: Inhibitory Postsynaptic Potentials 2. Zero hunger 6-Cyano-7-nitroquinoxaline-2 3-dione Neurons 0303 health sciences MESH: Kynurenic Acid Vagus Nerve MESH: Naphthalenes MESH: Excitatory Amino Acid Antagonists MESH: Glutamic Acid Endocannabinoid system [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Pyridazines MESH: Piperidines MESH: Benzoxazines Excitatory postsynaptic potential GABAergic Rimonabant MESH: Efferent Pathways Morpholines Glutamic Acid MESH: Morpholines Arachidonic Acids Tetrodotoxin Biology Neurotransmission Naphthalenes Inhibitory postsynaptic potential MESH: Calcium Signaling Efferent Pathways 03 medical and health sciences Cellular and Molecular Neuroscience Glutamatergic MESH: Mice Inbred C57BL MESH: Pyridazines MESH: Receptors Presynaptic Cannabinoid Receptor Modulators Animals MESH: Arachidonic Acids Calcium Signaling MESH: Excitatory Postsynaptic Potentials [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] MESH: Mice 030304 developmental biology Pharmacology Excitatory Postsynaptic Potentials MESH: Male MESH: Tetrodotoxin Vagus nerve Benzoxazines Mice Inbred C57BL Dorsal motor nucleus 2-Amino-5-phosphonovalerate Inhibitory Postsynaptic Potentials MESH: Brain Stem Pyrazoles MESH: 2-Amino-5-phosphonovalerate Capsaicin Neuroscience Excitatory Amino Acid Antagonists 030217 neurology & neurosurgery MESH: Pyrazoles Brain Stem Endocannabinoids |
| Zdroj: | Neuropharmacology Neuropharmacology, 2009, 56 (8), pp.1106-15. ⟨10.1016/j.neuropharm.2009.03.009⟩ Neuropharmacology, Elsevier, 2009, 56 (8), pp.1106-15. ⟨10.1016/j.neuropharm.2009.03.009⟩ |
| ISSN: | 0028-3908 |
| Popis: | International audience; Numerous studies, focused on the hypothalamus, have recently implicated endocannabinoids (EC) as orexigenic factors in the central control of food intake. However, the EC system is also highly expressed in the hindbrain autonomic integrator of food intake regulation, i.e. the dorsal vagal complex (DVC). Previous studies have shown that exogenous cannabinoids, by acting on cannabinoid 1 receptor (CB1R), suppress GABAergic and glutamatergic neuronal transmission in adult rat dorsal motor nucleus of the vagus nerve (DMNV), the principal efferent compartment of the DVC. However, no endogenous release of EC has been demonstrated in DVC to date. Using patch-clamp techniques on mouse coronal brainstem slices, we confirmed that both inhibitory and excitatory neurotransmission were depressed by WIN 55,212-2, a CB1R agonist. We demonstrated that DMNV neurons exhibited a rapid and reversible depolarization-induced suppression of electrically evoked GABAergic IPSCs (eIPSCs), classically known as DSI (depolarization-induced suppression of inhibition), while spontaneous or miniature IPSCs activity remained unaltered. Further, no depolarization-induced suppression of glutamatergic eEPSCs (DSE) occurred. Our results indicate that DSI was blocked by SR141716A (Rimonabant), a selective CB1R antagonist, and was dependent on calcium elevation in DMNV neurons, suggesting a release of EC in the DVC. Moreover, the analysis of the paired-pulse ratio, of the coefficient of variation and of the failure rate of eIPSCs support the fact that EC-mediated suppression of GABAergic inhibition takes place at the presynaptic level. These results show for the first time that DMNV neurons release EC in an activity-dependent manner, which in turn differentially regulates their inhibitory and excitatory synaptic inputs. |
| Databáze: | OpenAIRE |
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