Neurogenin3 Activation Is Not Sufficient to Direct Duct-to-Beta Cell Transdifferentiation in the Adult Pancreas*
| Autor: | Ping Guo, Xiangwei Xiao, Yousef El-Gohary, Krishna Prasadan, Iljana Gaffar, George K. Gittes, Chiyo Shiota, John Wiersch |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
endocrine system Succinimides Enteroendocrine cell Mice Transgenic Nerve Tissue Proteins Biology digestive system Biochemistry Neogenesis Mice Internal medicine Insulin-Secreting Cells medicine Basic Helix-Loop-Helix Transcription Factors Animals Progenitor cell Molecular Biology Fluorescent Dyes Pancreatic duct Transdifferentiation Pancreatic Ducts Cell Biology biology.organism_classification Fluoresceins Cell biology medicine.anatomical_structure Endocrinology Cell Transdifferentiation Beta cell Pancreas Developmental Biology |
| Popis: | It remains controversial whether adult pancreatic ducts harbor facultative beta cell progenitors. Because neurogenin3 (Ngn3) is a key determinant of pancreatic endocrine cell neogenesis during embryogenesis, many studies have also relied upon Ngn3 expression as evidence of beta cell neogenesis in adults. Recently, however, Ngn3 as a marker of adult beta cell neogenesis has been called into question by reports of Ngn3 expression in fully-developed beta cells. Nevertheless, direct evidence as to whether Ngn3 activation in adult pancreatic duct cells may lead to duct-to-beta cell transdifferentiation is lacking. Here we studied two models of Ngn3 activation in adult pancreatic duct cells (low-dose alloxan treatment and pancreatic duct ligation) and lineage-traced Ngn3-activated duct cells by labeling them through intraductal infusion with a cell-tagging dye, CFDA-SE No dye-labeled beta cells were found during the follow-up in either model, suggesting that activation of Ngn3 in duct cells is not sufficient to direct their transdifferentiation into beta cells. Therefore, Ngn3 activation in duct cells is not a signature for adult beta cell neogenesis. |
| Databáze: | OpenAIRE |
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