Comparative estrogenic activity of wine extracts and organochlorine pesticide residues in food
| Autor: | B Blankvoort, Ichen Chen, Kevin W. Gaido, Fen Wang, Stephen Safe, L Dohme, Kavita Ramamoorthy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Male Insecticides medicine.drug_class Health Toxicology and Mutagenesis Estrogen receptor Food Contamination Mice Inbred Strains Wine Estrogenic activity Toxicology Bioassays Toxaphene Organochlorine pesticide mixtures Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Hydrocarbons Chlorinated Tumor Cells Cultured medicine Wine extracts Animals Humans Bioassay Food science Toxicologie 030304 developmental biology 0303 health sciences Estradiol Pesticide residue Alcoholic Beverages Pesticide Residues Public Health Environmental and Occupational Health In vitro toxicology Estrogens Methoxychlor Diet Biochemistry chemistry Estrogen Enzyme Induction 030220 oncology & carcinogenesis Female Research Article |
| Zdroj: | Environmental Health Perspectives 106 (1998) Suppl. 6 Environmental Health Perspectives, 106(Suppl. 6), 1347-1351 Environmental Health Perspectives |
| ISSN: | 0091-6765 |
| Popis: | The human diet contains industrial-derived, endocrine-active chemicals and higher levels of naturally occurring compounds that modulate multiple endocrine pathways. Hazard and risk assessment of these mixtures is complicated by noadditive interactions between different endocrine-mediated responses. This study focused on estrogenic chemicals in the diet and compared the relative potencies or estrogen equivalents (EQs) of the daily consumption of xenoestrogenic organochlorine pesticides in food (2.44 micrograms/day) with the EQs in a single 200-ml glass of red cabernet wine. The reconstituted organochlorine mixture contained 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl)ethane, 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane, 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene, endosulfan-1, endosulfan-2, p,p'-methoxychlor, and toxaphene; the relative proportion of each chemical in the mixture resembled the composition reported in a recent U.S. Food and Drug Administration market basket survey. The following battery of in vitro 17 beta-estradiol (E2)-responsive bioassays were utilized in this study: competitive binding to mouse uterine estrogen receptor (ER); proliferation in T47D human breast cancer cells; luciferase (Luc) induction in human HepG2 cells transiently cotransfected with C3-Luc and the human ER, rat ER-alpha, or rat ER-beta; induction of chloramphenicol acetyltransferase (CAT) activity in MCF-7 human breast cancer cells transfected with E2-responsive cathepsin D-CAT or creatine kinase B-CAT plasmids. For these seven in vitro assays, the calculated EQs in extracts from 200 ml of red cabernet wine varied from 0.15 to 3.68 micrograms/day. In contrast, EQs for consumption of organochlorine pesticides (2.44 micrograms/day) varied from nondetectable to 1.24 ng/day. Based on results of the in vitro bioassays, organochlorine pesticides in food contribute minimally to dietary EQ intake. Images Figure 3 |
| Databáze: | OpenAIRE |
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