Hydrogen peroxide-induced proline and metabolic pathway of its accumulation in maize seedlings
| Autor: | Shuang-Long Yang, Shan-Shan Lan, Ming Gong |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Proline
Physiology Ornithine aminotransferase Glutamate dehydrogenase Hydrogen Peroxide Plant Science Biology Ornithine Zea mays Ligases Arginase Metabolic pathway chemistry.chemical_compound Proline dehydrogenase Glutamate Dehydrogenase Biosynthesis chemistry Biochemistry Gene Expression Regulation Plant Seedlings Cotyledon Agronomy and Crop Science Plant Proteins |
| Zdroj: | Journal of Plant Physiology. 166:1694-1699 |
| ISSN: | 0176-1617 |
| Popis: | Summary Exogenous H 2 O 2 treatment led to a significant accumulation of proline in coleoptiles and radicles of maize seedlings. It also induced an almost immediate and rapid increase of activity of the key enzymes Δ 1 -pyrroline-5-carboxylate synthetase and glutamate dehydrogenase of the glutamate pathway of proline biosynthesis and an up-regulation of Δ 1 -pyrroline-5-carboxylate synthetase gene expression. Activities of the key enzymes arginase and ornithine aminotransferase of the ornithine pathway of proline biosynthesis increased only after 12 h of H 2 O 2 treatment. Furthermore, the H 2 O 2 treatment caused an early decrease of the activity of proline dehydrogenase, a key enzyme of proline degradation. These results indicate that H 2 O 2 might be involved in signal transduction events, leading to proline accumulation in maize seedlings, and that the H 2 O 2 -induced proline accumulation is a combined result of the sequential activation of the glutamate and ornithine pathways of proline biosynthesis and the simultaneous inhibition of proline degradation by H 2 O 2 . |
| Databáze: | OpenAIRE |
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