Molecular Characterization ofLeishmaniaParasites in Giemsa-Stained Slides from Cases of Human Cutaneous and Visceral Leishmaniasis, Eastern Algeria
| Autor: | Hejer Souguir Omrani, Alia Yaacoub, Farida Saadni, Jihene Bettaieb, Roukaya Mansouri, Souheila Guerbouj, Yusr Saadi Ben Aoun, Nadia Beldi, Ikram Guizani |
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| Přispěvatelé: | Laboratoire d'Epidémiologie Moléculaire et de Pathologie Expérimentale Appliquée aux Maladies Infectieuses (LR11IPT04), Université de Tunis El Manar (UTM)-Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Badji Mokhtar-Annaba University, Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Leishmania tropica diagnosis ITS1 030231 tropical medicine 030106 microbiology Leishmaniasis Cutaneous Azure Stains Polymerase Chain Reaction Microbiology Giemsa stain PCR-RFLP 03 medical and health sciences 0302 clinical medicine Bone Marrow Zoonoses Virology DNA Ribosomal Spacer parasitic diseases medicine Animals Humans Leishmania major leishmaniasis Giemsa-stained slides Skin Leishmania biology mini-exon Leishmaniasis medicine.disease biology.organism_classification DNA extraction Molecular biology 3. Good health Infectious Diseases Visceral leishmaniasis Algeria Leishmaniasis Visceral Leishmania infantum [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Polymorphism Restriction Fragment Length |
| Zdroj: | Vector-Borne and Zoonotic Diseases Vector-Borne and Zoonotic Diseases, Mary Ann Liebert, 2017, 17 (6), pp.416-424. ⟨10.1089/vbz.2016.2071⟩ |
| ISSN: | 1557-7759 1530-3667 |
| DOI: | 10.1089/vbz.2016.2071 |
| Popis: | International audience; BACKGROUND:In Algeria, visceral leishmaniasis (VL) is due to Leishmania (L.) infantum, while three cutaneous forms (CL) are caused by Leishmania major, Leishmania tropica and Leishmania infantum. In this study, the use of Giemsa-stained slides was evaluated with two PCR techniques, in Eastern Algeria.MATERIALS AND METHODS:A total of 136 samples corresponding to 100 CL smears (skin scrapings) and 36 VL slides (bone marrow aspirates) collected from 2008 to 2014 were tested. Upon DNA extraction, two PCRs were used to amplify the ribosomal Internal Transcribed Spacer 1 (ITS1) and mini-exon genes. Amplified products were digested (PCR-RFLP) and profiles analyzed for Leishmania species identification. A statistical analysis was also performed.RESULTS:ITS1-PCR was found significantly more sensitive than mini-exon-PCR (77.95% positives vs. 67.65%; p = 0.001). Comparison of PCR positivity showed statistically significant differences between old and recently prepared slides suggesting a better use of recent slides in PCR analyses. For species identification, PCR-restriction fragment length polymorphism (RFLP) results of ITS1 and mini-exon were concordant. L. infantum was identified from VL cases and L. infantum, L. major, and L. tropica from CL ones. According to geographical origin, L. infantum was found in North-Eastern provinces, while L. major was distributed from the North to the Center-East of Algeria. Interestingly, two L. tropica samples were identified in Annaba, located far North-East Algeria.CONCLUSION:Distribution of leishmaniasis in Eastern parts of Algeria, besides finding of L. tropica in the far North, is in this study described for the first time using molecular tools, thus confirming the usefulness of slides for PCR identification of Leishmania parasites in retrospective epidemiological investigations. |
| Databáze: | OpenAIRE |
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