Intrahippocampal infusion of an inhibitor of protein kinase A separates short- from long-term memory
| Autor: | Jorge H. Medina, Daniela M. Barros, Luciana A. Izquierdo, Ivan Antonio Izquierdo, Monica R. M. Vianna |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Indoles medicine.medical_treatment Carbazoles Amnesia Inhibitory postsynaptic potential Hippocampus Injections chemistry.chemical_compound Memory Internal medicine Avoidance Learning medicine Animals Pyrroles Enzyme Inhibitors Rats Wistar Protein kinase A Saline Pharmacology chemistry.chemical_classification Behavior Animal Dose-Response Relationship Drug Long-term memory KT5720 Cyclic AMP-Dependent Protein Kinases Rats Psychiatry and Mental health Dose–response relationship Memory Short-Term Endocrinology Enzyme chemistry Amnesia Retrograde medicine.symptom |
| Zdroj: | Behavioural Pharmacology. 10:223-227 |
| ISSN: | 0955-8810 |
| DOI: | 10.1097/00008877-199903000-00011 |
| Popis: | Rats implanted bilaterally with cannulae in the CA1 region of the dorsal hippocampus were trained in one-trial step-down inhibitory (passive) avoidance, and tested for short- and long-term memory of this task at 1.5-3.0 and at 24 h from training, respectively. At various times after training (0, 22, 45, 90, 135 or 175 min) they received a 0.5 microl infusion of the protein kinase A (PKA) inhibitor, KT5720 (0.1 or 0.5 microg), or of its vehicle (20% dimethylsulfoxide in saline). At the higher dose, KT5720 inhibited PKA activity by 90%. KT5720 blocked long-term memory (LTM) when given either 0 or 175 min posttraining, and short-term memory (STM) when given 0, 22, 45 or 90 min post-training. Therefore, PKA plays a different role in the process of formation of the two types of memory. Its role in LTM may be related to the peak of PKA activity, and to the levels of its substrate, nuclear P-CREB, that have been described in a previous paper to occur at 0 and again at 3 h after training. The role of PKA in STM may well involve other substrates of the enzyme. This finding points to a cleavage between the mechanisms of STM and LTM formation. |
| Databáze: | OpenAIRE |
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