Dose-dependent transcriptome changes by metal ores on a human acute lymphoblastic leukemia cell line
| Autor: | Simon S. Wong, Gary Ridenour, Cynthia D. Fastje, Paul R. Sheppard, Nina N. Sun, Stephanie J Macdonald, Juanita Hyde, Mark L. Witten |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
0301 basic medicine
Childhood leukemia T-Lymphocytes Health Toxicology and Mutagenesis 030106 microbiology Down-Regulation Gene Expression Environmental pollution In Vitro Techniques Biology Toxicology Mining Tungsten Transcriptome 03 medical and health sciences Cell Line Tumor Metals Heavy medicine Humans Metallothionein Incubation Cell Proliferation Oligonucleotide Array Sequence Analysis Dose-Response Relationship Drug Cell growth Metallurgy Arizona Public Health Environmental and Occupational Health Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Molecular biology Up-Regulation Leukemia 030104 developmental biology Cell culture RNA Environmental Pollution Nevada |
| Zdroj: | Toxicology and Industrial Health. 19:157-163 |
| ISSN: | 1477-0393 0748-2337 |
| DOI: | 10.1191/0748233703th185oa |
| Popis: | The increased morbidity of childhood leukemia in Fallon, Nevada and Sierra Vista, Arizona has prompted great health concern. The main characteristic that these two towns share is the environmental pollution attributed to metal ore from abandoned mining operations. Consequently, we have investigated the transcriptome effects of metal ores from these endemic areas using a human T-cell acute lymphoblastic leukemia cell line (T-ALL). Metal ore from Fallon significantly increased cell growth after 24, 48 and 72 h of incubation at 1.5 mg/mL concentration, as measured by trypan-blue. Sierra Vista ore significantly increased cell growth with 0.15 and 1.5 mg/mL following 72 h of incubation. From human cDNA microarray, results indicate that in total, eight genes, mostly metallothionein (MT) genes, were up-regulated and 10 genes were down-regulated following treatment of the T-ALL cells with 0.15 and 1.5 mg/mL of metal ores at 72 h, in comparison with untreated cells. Twenty-eight metals of both ores were quantified and their presence may be associated with the cell growth rate and dose-dependent activation of transcriptomes in immature T-cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|