Temporal evolution of single-cell transcriptomes of Drosophila olfactory projection neurons
| Autor: | Anthony Xie, Sai Saroja Kolluru, Qijing Xie, Anay R Reddy, Felix Horns, Liqun Luo, Stephen R. Quake, Zhuoran Li, David Vacek, David J. Luginbuhl, Hongjie Li, Maria Brbic, Sayeh Kohani, Jiefu Li, Chuanyun Xu, Tongchao Li, Colleen N McLaughlin, Jure Leskovec, Robert C. Jones |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Brain development
Future studies QH301-705.5 media_common.quotation_subject Science Cell Biology General Biochemistry Genetics and Molecular Biology neural development Transcriptome medicine Metamorphosis Biology (General) Projection (set theory) Drosophila media_common General Immunology and Microbiology General Neuroscience RNA sequencing General Medicine olfactory circuit single-cell biology.organism_classification wiring medicine.anatomical_structure nervous system Medicine Neural development Neuroscience transcriptome |
| Zdroj: | eLife, Vol 10 (2021) |
| Popis: | Neurons undergo substantial morphological and functional changes during development to form precise synaptic connections and acquire specific physiological properties. What are the underlying transcriptomic bases? Here, we obtained the single-cell transcriptomes ofDrosophilaolfactory projection neurons (PNs) at four developmental stages. We decoded the identity of 21 transcriptomic clusters corresponding to 20 PN types and developed methods to match transcriptomic clusters representing the same PN type across development. We discovered that PN transcriptomes reflect unique biological processes unfolding at each stage—neurite growth and pruning during metamorphosis at an early pupal stage; peaked transcriptomic diversity during olfactory circuit assembly at mid-pupal stages; and neuronal signaling in adults. At early developmental stages, PN types with adjacent birth order share similar transcriptomes. Together, our work reveals principles of cellular diversity during brain development and provides a resource for future studies of neural development in PNs and other neuronal types. |
| Databáze: | OpenAIRE |
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