MicroRNA-181a and its target Smad 7 as potential biomarkers for tracking child acute lymphoblastic leukemia
| Autor: | Randa Ali-Labib, Eman Khairy, Safinaz Adel Elhabashy, Manal L. Louka, Marwa Nabhan, Fathy Tash |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Hepatosplenomegaly Enzyme-Linked Immunosorbent Assay SMAD law.invention Smad7 Protein Transforming Growth Factor beta1 03 medical and health sciences 0302 clinical medicine law Cyclin-dependent kinase Genetics medicine Biomarkers Tumor Humans Child integumentary system Oncogene biology Gene Expression Regulation Leukemic Infant Reproducibility of Results General Medicine Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Leukemia MicroRNAs 030104 developmental biology Real-time polymerase chain reaction Phenotype ROC Curve 030220 oncology & carcinogenesis Case-Control Studies Child Preschool Immunology biology.protein Suppressor Female medicine.symptom Signal transduction |
| Zdroj: | Gene. 628 |
| ISSN: | 1879-0038 |
| Popis: | Acute lymphoblastic leukemia (ALL) is the most common pediatric hematologic tumor. MiR-181a was expected to have a role in the development of hematological malignancies; it might act as tumor suppressor or oncogene. Smad7 was selected as miR-181a target pair. It is a negative regulator for the TGF-β1 signaling pathway. In this study, relative expression levels of miR-181a by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), both Smad 7 and TGF-β1 proteins levels by enzyme linked immunosorbent assay (ELISA) were all measured in serum of 60 child, 30 with ALL and 30 age and sex matched healthy child as control group. MiR-181a expression showed highly significant decrease; plus a significant increase and decrease of Smad7 and TGF-β1 protein levels respectively, in serum samples of ALL as compared to control group. MiR-181a expression achieved a highly significant positive and a significant negative correlation with TGF-β1 and Smad7 respectively. Furthermore, the levels of Smad7 and TGF-β1 were negatively correlated with each other (p |
| Databáze: | OpenAIRE |
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