Identification by MicroRNA Analysis of Environmental Risk Factors Bearing Pathogenic Relevance in Non-Smoker Lung Cancer
Autor: | Izzotti, Alberto, Vargas, Gabriela Coronel, Pulliero, Alessandra, Coco, Simona, Colarossi, Cristina, Blanco, Giuseppina, Agodi, Antonella, Barchitta, Martina, Maugeri, Andrea, Workers, CT-ME-EN Cancer Registry Workers CT-ME-EN Cancer Registry, Conti, Gea Oliveri, Ferrante, Margherita, Sciacca, Salvatore |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Microarray Medicine (miscellaneous) Environmental pollution environmental risk factors Article 03 medical and health sciences 0302 clinical medicine Internal medicine microRNA DNA adduct medicine Lung cancer no-smokers lung cancer Environmental Carcinogen Lung business.industry Cancer DNA adducts medicine.disease respiratory tract diseases 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Medicine business Environmental risk factors MicroRNA No-smokers lung cancer |
Zdroj: | Journal of Personalized Medicine Volume 11 Issue 7 Journal of Personalized Medicine, Vol 11, Iss 666, p 666 (2021) |
ISSN: | 2075-4426 |
DOI: | 10.3390/jpm11070666 |
Popis: | MicroRNA and DNA adduct biomarkers may be used to identify the contribution of environmental pollution to some types of cancers. The aim of this study was to use integrated DNA adducts and microRNAs analyses to study retrospectively the contribution of exposures to environmental carcinogens to lung cancer in 64 non-smokers living in Sicily and Catania city near to the Etna volcano. MicroRNAs were extracted from cancer lung biopsies, and from the surrounding lung normal tissue. The expression of 2549 human microRNAs was analyzed by microarray. Benzo(a)Pyrene-DNA adducts levels were analyzed in the patients’ blood by HPLC−fluorescence detection. Correlations between tetrols and environmental exposures were calculated using Pearson coefficients and regression variable plots. Compared with the healthy tissue, 273 microRNAs were downregulated in lung cancer. Tetrols levels were inversely related both with the distance from Etna and years since smoking cessation, but they were not significantly correlated to environmental exposures. The analysis of the microRNA environmental signatures indicates the contribution of environmental factors to the analyzed lung cancers in the following decreasing rank: (a) car traffic, (b) passive smoke, (c) radon, and (d) volcano ashes. These results provide evidence that microRNA analysis can be used to retrospectively investigate the contribution of environmental factors in human lung cancer occurring in non-smokers. |
Databáze: | OpenAIRE |
Externí odkaz: |